Immunomodulatory activity of ethanolic extract of Vitis vinifera L. in laboratory animal

Globally, Vitis vinifera L. is used to make wine. It is also used to cure skin and eye diseases. Ripe grapes were used to cure cancer, cholera, smallpox, kidney and liver diseases. In the present study, phytochemical analysis and immunomodulatory activity of ethanolic extract of the fruit of V. vinifera were investigated. Laboratory-based experimental methods were used to evaluate immunomodulatory activity by dividing animals into six different groups, i.e., control, levamisole (Std-1), cyclophosphamide (Std-2), EVV150, EVV300 and EVV450 mg/kg, comprising six animals in each. The immunomodulatory activities were determined by using the carbon clearance test, delayed type hypersensitivity (DTH) test, neutrophil adhesion test, haemagglutination antibody (HA) titer response and T cell population assay. The response produced by oral administration of ethanolic extracts of the fruit of V. vinifera showed a significant dose-dependent increase in phagocytic activity, DTH response, neutrophil adhesion response, augmentation of humoral immune response to sheep red blood cells and an increase in T cell population response. Finally, the results concluded that ethanolic extract of the fruit of V. vinifera caused a significant immunostimulatory effect on both the cell-mediated and humoral immune systems in the Wistar albino rats.