Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes

被引：11
|
作者：
Zhong XingYu [1 ,2 ]
Tian YuQing [1 ]
Niu GuoQing [1 ]
Tan HuaRong [1 ]
机构：
[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
SCIENCE CHINA-LIFE SCIENCES | 2013年 / 56卷 / 07期
基金：
中国国家自然科学基金;
关键词：
secondary metabolite; gene cluster; assemble; features; draft genome; GENOME SEQUENCE; IDENTIFICATION; ANNOTATION; BACTERIAL; PRODUCTS;
D O I：
10.1007/s11427-013-4506-0
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A draft genome sequence of Streptomyces ansochromogenes 7100 was generated using 454 sequencing technology. In combination with local BLAST searches and gap filling techniques, a comprehensive antiSMASH-based method was adopted to assemble the secondary metabolite biosynthetic gene clusters in the draft genome of S. ansochromogenes. A total of at least 35 putative gene clusters were identified and assembled. Transcriptional analysis showed that 20 of the 35 gene clusters were expressed in either or all of the three different media tested, whereas the other 15 gene clusters were silent in all three different media. This study provides a comprehensive method to identify and assemble secondary metabolite biosynthetic gene clusters in draft genomes of Streptomyces, and will significantly promote functional studies of these secondary metabolite biosynthetic gene clusters.
引用
收藏
页码：609 / 618
页数：10
相关论文
共 50 条
  • [41] In silico prediction and characterization of secondary metabolite biosynthetic gene clusters in the wheat pathogen Zymoseptoria tritici
    Cairns, Timothy
    Meyer, Vera
    BMC GENOMICS, 2017, 18
  • [42] Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters
    Kallifidas, Dimitris
    Jiang, Guangde
    Ding, Yousong
    Luesch, Hendrik
    MICROBIAL CELL FACTORIES, 2018, 17
  • [43] Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters
    Li, Hui
    Gao, Sheng
    Shi, Sanyuan
    Zhao, Xiaomin
    Ye, Haoyu
    Luo, Yunzi
    SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2024, 9 (03) : 600 - 608
  • [44] Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters
    Dimitris Kallifidas
    Guangde Jiang
    Yousong Ding
    Hendrik Luesch
    Microbial Cell Factories, 17
  • [45] Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces bacteria
    Belknap, Kaitlyn C.
    Park, Cooper J.
    Barth, Brian M.
    Andam, Cheryl P.
    SCIENTIFIC REPORTS, 2020, 10 (01)
  • [46] Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production
    Guojian Liao
    Jine Li
    Lei Li
    Haihua Yang
    Yuqing Tian
    Huarong Tan
    Microbial Cell Factories, 9
  • [47] Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces bacteria
    Kaitlyn C. Belknap
    Cooper J. Park
    Brian M. Barth
    Cheryl P. Andam
    Scientific Reports, 10
  • [48] Application of serine integrases for secondary metabolite pathway assembly in Streptomyces
    Ga, Hong
    Taylor, Gabrielle
    Evans, Stephanie K.
    Fogg, Paul C. M.
    Smith, Margaret C. M.
    SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2020, 5 (02) : 111 - 119
  • [49] A novel Streptomyces gene, samR, with different effects on differentiation of Streptomyces ansochromogenes and Streptomyces coelicolor
    Huarong Tan
    Yuqing Tian
    Haihua Yang
    Gang Liu
    Liping Nie
    Archives of Microbiology, 2002, 177 : 274 - 278
  • [50] Current status of secondary metabolite pathways linked to their related biosynthetic gene clusters in Aspergillus section Nigri
    Wang, Xinhui
    Jarmusch, Scott A.
    Frisvad, Jens C.
    Larsen, Thomas O.
    NATURAL PRODUCT REPORTS, 2023, 40 (02) : 237 - 274
12345