Etoposide and Hypoxia Do Not Activate Apoptosis of Multipotent Mesenchymal Stromal Cells In Vitro

被引：9
|
作者：
Rylova, J. V. [1 ,2 ]
Andreeva, E. R. [1 ]
Gogvadze, V. G. [2 ,3 ]
Zhivotovsky, B. D. [2 ,3 ]
Buravkova, L. B. [1 ,2 ]
机构：
[1] Russian Acad Sci, Inst Biomed Problems, Moscow 117901, Russia
[2] Moscow MV Lomonosov State Univ, Moscow 117234, Russia
[3] Karolinska Inst, Stockholm, Sweden
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2012年 / 154卷 / 01期
关键词：
multipotent mesenchymal stromal cells from adipose tissue; etoposide; hypoxia; HUMAN ADIPOSE-TISSUE; STEM-CELLS;
D O I：
10.1007/s10517-012-1895-1
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Incubation of multipotent mesenchymal stromal cells from human adipose tissue with etoposide for 24, 48, and 72 h under standard conditions (20% O-2), at "physiological" oxygen content (5% O-2), and under hypoxic conditions (1% O-2) did not induce cell apoptosis and only slightly increased the number of necrotic cells. The absence of growth factors in the culture medium did not potentiate the damaging effect of etoposide on multipotent mesenchymal stromal cells under standard and hypoxic conditions. We concluded that etoposide produced no pro-apoptotic effect on multipotent mesenchymal stromal cells from the human adipose tissue.
引用
收藏
页码：141 / 144
页数：4
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