Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody

被引：3

作者：

Nikolelis, Dimitrios P. ^{[1
]}

Psaroudakis, Nikolas ^{[3
]}

Michaloliakos, Antonis I. ^{[1
]}

Nikoleli, Georgia-Paraskevi ^{[2
]}

Scoullos, Michael ^{[1
]}

机构：

[1] Univ Athens, Dept Chem, Environm Chem Lab, GR-15771 Athens, Greece

[2] Natl Tech Univ Athens, Lab Inorgan & Analyt Chem, Sch Chem Engn, Dept 1, GR-15780 Athens, Greece

[3] Univ Athens, Dept Chem, Lab Inorgan Chem, GR-15771 Athens, Greece

来源：

CENTRAL EUROPEAN JOURNAL OF CHEMISTRY | 2013年 / 11卷 / 02期

关键词：

Congener; 77; Stabilized lipid films; Biosensor; Flow injection; Antibody regeneration; MICROWAVE-ASSISTED EXTRACTION; POLYCHLORINATED-BIPHENYLS; PCBS;

D O I：

10.2478/s11532-012-0157-4

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3',4,4' tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10(-8) to 10(-5) M, with a detection limit of ca. 10(-8) M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5.

引用

页码：320 / 323

页数：4

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