Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay

被引：12

作者：

Fukushima, Kazuko Y. ^{[1
]}

Yanagihara, Katsunori ^{[1
]}

Hirakata, Yoichi ^{[2
]}

Sugahara, Kazuyuki ^{[1
]}

Morinaga, Yoshitomo ^{[1
,2
]}

Kohno, Shigeru ^{[2
]}

Kamihira, Shimeru ^{[1
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Dept Lab Med, Nagasaki 8528501, Japan

[2] Nagasaki Univ, Grad Sch Biomed Sci, Dept Internal Med 2, Nagasaki 8528501, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 07期

关键词：

D O I：

10.1128/JCM.00051-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel- based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.

引用

页码：2384 / 2388

页数：5

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