Analysis of interleukin-4 and interleukin-10 and their association with the lymphocytic infiltrate in the small intestine of patients with coeliac disease

被引:46
|
作者
Beckett, CG
DellOlio, D
Kontakou, M
Przemioslo, RT
RosenBronson, S
Ciclitira, PJ
机构
[1] UNITED MED & DENT SCH,GASTROENTEROL UNIT,LONDON SE1 7EH,ENGLAND
[2] GEORGETOWN UNIV,WASHINGTON,DC
关键词
coeliac disease; interleukin-4; interleukin-10; lymphocytes;
D O I
10.1136/gut.39.6.818
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background-Concentrations of proinflammatory cytokines are raised in the small intestine of patients with coeliac disease after ingestion of gluten but there are equivalent data an interleukin-4 (IL-4) and interleukin-10 (IL-10) producing cells, These cytokines are known to exert important regulatory effects on proinflammatory cytokine production from lymphocytes and macrophages. Aims-To investigate whether there Is a primary deficiency of IL-4 and IL-10 producing cells and their site of production in the small intestine of patients with coeliac disease in relation to the changes in inflammatory cell infiltrate. Patients-Jejunal biopsy specimens from patients with coeliac disease (11 untreated, 10 treated) and nine disease controls were studied. Methods-Immunohistochemical staining of sections for IL-4 and IL-10 cytokines and the cell phenotypic markers CD3 (T lymphocytes) and CD45 (total inflammatory cell infiltrate) was carried out using monoclonal antibodies, Expression of IL-4 and IL-10 messenger RNA was detected by in situ hybridisation with oligonucleotide probe cocktails for each cytokine, Results-IL-4 and IL-10 mRNA and protein were detected in the lamina propria of treated and untreated coeliac patients and disease controls but not in the epithelium. A significant increase in the number of CD45 (p<0 . 005) and CD3 (p<0 . 05) positive cells was found in the lamina propria of patients with untreated coeliac disease compared with treated coeliac patients and disease controls but there were no differences in IL-4 or IL-10 between these groups with either method. Conclusions-There is no primary deficiency of IL-4 and IL-10 producing cells in the small intestine of patients with coeliac disease, Detectable concentrations Georgetown of IL-4 and IL-10 were found in control University, patients which suggests that these cytokines are involved in normal mucosal immunoregulation. The increased number of T lymphocytes but not. IL-4 or IL-10 producing cells in the lamina propria of patients with untreated than in those with treated disease suggests not only that the lamina propria is the major mucosal compartment for cytokine production but that newly recruited mucosal T lymphocytes are directed to a predominant Th1 and not a Th2 cytokine response in coeliac patients on a diet containing gluten.
引用
收藏
页码:818 / 823
页数:6
相关论文
共 50 条
  • [21] Increased interleukin-10 and interleukin-4 mRNA expression in gut mucosa from patients with allergic asthma
    Lamblin, C
    Desreumaux, P
    Colombel, JF
    Tonnell, AB
    Wallert, B
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2000, 105 (01) : S149 - S149
  • [22] IMMUNOMODULATING TREATMENT WITH LOW DOSE INTERLEUKIN-4, INTERLEUKIN-10 AND INTERLEUKIN-11 IN PSORIASIS VULGARIS
    Roberti, M. L.
    Ricottini, L.
    Capponi, A.
    Sclauzero, E.
    Vicenti, P.
    Fiorentini, E.
    Savoia, C.
    Scornavacca, G.
    Brazioli, D.
    Gaio, L.
    Giannetti, R.
    Ignazzi, C.
    Meloni, G.
    Chinni, L. M.
    JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS, 2014, 28 (01): : 133 - 139
  • [23] Interleukin-1 signaling in mouse astrocytes involves Akt: a study with interleukin-4 and interleukin-10
    Pousset, F
    Dantzer, R
    Kelley, KW
    Parnet, P
    EUROPEAN CYTOKINE NETWORK, 2000, 11 (03) : 427 - 434
  • [24] Genetic association of interleukin-4, interleukin-10, and transforming growth factor-β gene polymorphism with allograft function in renal transplant patients
    Amirzargar, M.
    Yavangi, M.
    Basiri, A.
    Moghadam, S. H.
    Khosravi, F.
    Solgi, G.
    Gholiaf, M.
    Khoshkho, F.
    Dadaras, F.
    Mahmmodi, M.
    Ansaripour, B.
    Amirzargar, A.
    Nikbin, B.
    TRANSPLANTATION PROCEEDINGS, 2007, 39 (04) : 954 - 957
  • [25] Differential effects of interleukin-4 and interleukin-10 on nitric oxide production by murine macrophages
    Y. Nemoto
    T. Otsuka
    H. Niiro
    K. Izuhara
    K. Yamaoka
    H. Nakashima
    Y. Niho
    Inflammation Research, 1999, 48 : 643 - 650
  • [26] Interleukin-4 and interleukin-10 prevent blood-induced cartilage damage in vitro
    Van Meegeren, M.
    Jansen, N.
    Roosendaal, G.
    Lafeber, F.
    HAEMOPHILIA, 2011, 17 (02) : 372 - 372
  • [27] Interleukin-17 and Interleukin-10 Association with Disease Progression in Schizophrenia
    Chenniappan, Raghavi
    Nandeesha, Hanumanthappa
    Kattimani, Shivanand
    Nanjaiah, Nandakumar Dalavaikodihalli
    ANNALS OF NEUROSCIENCES, 2020, 27 (01) : 24 - 28
  • [28] Interleukin-4, interleukin-10, and soluble tumor necrosis factor receptors in cord blood
    Hata, T
    Kawamura, T
    Fujiwaki, R
    Aoki, S
    Hata, K
    Inada, K
    GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 1997, 43 (03) : 155 - 157
  • [29] Interleukin-10 but not interleukin-4 suppressed an in vitro model for human proliferative synovitis.
    Takahashi, K
    Yamada, H
    Morluchi, E
    Ishii, O
    Ohya, N
    Kikukawa, T
    Ichikawa, Y
    ARTHRITIS AND RHEUMATISM, 1996, 39 (09): : 602 - 602
  • [30] Opposite effects of interleukin-4 and interleukin-10 on nitric oxide production in murine macrophages
    Maru, A
    Jackson, SK
    MEDIATORS OF INFLAMMATION, 1996, 5 (02) : 110 - 112