This study examined the effects of precooling via ice ingestion on female cycling performance in hot, humid conditions. Ten female endurance athletes, mean age (28 +/- 6 y), height (167.6 +/- 6.5 cm) and body-mass (68.0 +/- 11.5 kg) participated-in the study. Participants completed an 800 kJ cycle time-trial in hot, humid conditions (34.9 +/- 0.3 degrees C, 49.8 +/- 3.5% RH). This was preceded by the consumption of 7 g.kg(-1) of crushed ice (ICE) or water (CON). There was no difference in performance time (CON 3851 +/- 449 s; ICE 3767 +/- 465 s), oxygen consumption (CON 41.6 +/- 7.0 ml.kg.min(-1); ICE 42.4 +/- 6.0 ml.kg.min(-1)) or respiratory exchange ratio (CON 0.88 +/- 0.05; ICE 0.90 +/- 0.06) between conditions (p >.05, d < 0.5). Core and skin temperature following the precooling period were lower in ICE (T-c 36.4 +/- 0.4 degrees C; T-sk 31.6 +/- 1.2 degrees C) compared with CON (T-c 37.1 +/- 0.4 degrees C; T-sk 32.4 +/- 0.7 degrees C) and remained lower until the 100 kJ mark of the cycle time-trial (p <.05, d > 1.0). Sweat onset occurred earlier in CON (228 +/- 113 s) compared with ICE (411 +/- 156 s) (p <.05, d= 1.63). Mean thermal sensation (CON 1.8 +/- 2.0; ICE 1.2 +/- 2.5, p <.05, d = 2.51), perceived exertion (CON 15.3 +/- 2.9; ICE 14.9 +/- 3.0, p <.05, d = 0.38) and perceived thirst (CON 5.6 +/- 2.2; ICE 4.6 +/- 2.4, p <.05, d = 0.98) were lower in ICE compared with CON. Crushed ice ingestion did not improve cycling performance in females, although perceptual responses were reduced.