Synthesis, self-assembling and gene delivery potential of a novel highly unsaturated, conjugated cationic phospholipid

被引：5
|
作者：
Opstad, Christer L. [1 ]
Sliwka, Hans-Richard [1 ]
Partali, Vassilia [1 ]
Elgsaeter, Arnljot [2 ]
Leopold, Philip [3 ]
Jubeli, Emile [4 ,5 ]
Khalique, Nada Abdul [4 ,5 ]
Raju, Liji [4 ,5 ]
Pungente, Michael D. [5 ,6 ]
机构：
[1] Norwegian Univ Sci & Technol, Dept Chem, N-7491 Trondheim, Norway
[2] Norwegian Univ Sci & Technol, Dept Phys, N-7491 Trondheim, Norway
[3] Stevens Inst Technol, Dept Chem Chem Biol & Biomed Engn, Hoboken, NJ 07030 USA
[4] Weill Cornell Med Coll, Div Res, Doha, Qatar
[5] Educ City, Doha, Qatar
[6] Weill Cornell Med Coll, Premed Unit, Doha, Qatar
来源：
CHEMISTRY AND PHYSICS OF LIPIDS | 2013年 / 170卷
关键词：
Carotenoids; Unsaturated cationic phospholipid; Self-assembling; Gene transfer; SURFACE-PROPERTIES; HYDROPHILIC CAROTENOIDS; COMBINATION; AGGREGATION; TROLOX; LIPIDS; CHAIN; ACID;
D O I：
10.1016/j.chemphyslip.2013.03.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The synthesis and self-assembling properties of a model compound in a new class of cationic phospholipids with a highly unsaturated conjugated fatty acid are described. In addition, the potential of this new lipid as a nucleic acid carrier was evaluated through lipoplex formulations employing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as helper lipid with and without the polycationic peptide protamine, together with a plasmid DNA (pDNA). Lipoplexes composed of this novel unsaturated lipid exhibited pDNA binding and protection from DNase I degradation when formulated with protamine. The new cationic lipid revealed transfection efficiency comparable to the commercial reference 1,2-dimyristoyl-sn-glycero-3-ethylphophocholine (EPC) in Chinese hamster ovary-K1 (CHO-K1) cells and performed equally to the standard reference Lipofectamine 2000 when the formulation included protamine. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：65 / 73
页数：9
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