Restoration of chondrogenic properties of degenerative nucleus pulposus cells by repeated co-culture with adipose-derived stem cells

被引：4
|
作者：
Kim, Jin-Su [1 ,2 ]
Kwon, Daegi [1 ]
Cha, Byung-Hyun [1 ]
Moon, Bo Kyung [1 ]
Jeong, Younghyen [3 ]
Han, In-Bo [4 ]
Park, Hansoo [3 ]
Lee, Soo-Hong [1 ]
机构：
[1] CHA Univ, Dept Biomed Sci, 335 Pangyo Ro, Seongnam Si 13488, Gyeonggi Do, South Korea
[2] TJC LIFE Co Ltd, R&D Ctr, 473-3 Bangbae Dong, Seoul 06698, South Korea
[3] Chung Ang Univ, Sch Integrat Engn, 84 Heukseok Ro, Seoul 06974, South Korea
[4] CHA Univ, Bundang Med Ctr, Dept Neurosurg, Seongnam Si 13496, Gyeonggi Do, South Korea
来源：
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | 2018年 / 60卷
基金：
新加坡国家研究基金会;
关键词：
Repeated co-culture; Chondrogenic differentiation; Degenerated; Nucleus pulposus cell; INTERVERTEBRAL DISC; STROMAL CELLS; TISSUE; DIFFERENTIATION; REGENERATION; CHONDROCYTES; MODULATION; SYSTEM; PROGRESSION; PHENOTYPE;
D O I：
10.1016/j.jiec.2017.11.004
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We used porous membranes for co-culturing adipose-derived stem cells (ASCs) with nucleus pulposus cells (NPCs) to investigate whether repeated co-cultures restored the chondrogenic properties of degenerative NPCs (dNPCs). Human dNPCs were co-cultured with ASCs for 1 week and co-cultured again with new ASCs for 1 week. The expression levels of chondrogenic genes and GAGs were significantly higher in second co-cultured dNPCs than those in the first co-cultured dNPCs and in dNPCs cultured alone, respectively. After six weeks of in vivo transplantation, compared to first co-cultured dNPCs, second co-cultured dNPCs showed a significantly higher cartilage tissue formation. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：185 / 191
页数：7
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