Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests

被引：15

作者：

Chiu, Yi-Jen ^{[1
,2
]}

Nam, Mun-Kit ^{[3
]}

Tsai, Yueh-Ting ^{[3
]}

Huang, Chun-Chi ^{[1
,2
]}

Tsai, Cheng-Chih ^{[1
]}

机构：

[1] Hungkuang Univ, Dept Food Sci & Technol, Taichung 43302, Taiwan

[2] New Bellus Enterprises Co Ltd, Tainan 72042, Taiwan

[3] Super Lab Ltd, New Taipei 24890, Taiwan

来源：

SCIENTIFIC WORLD JOURNAL | 2013年

关键词：

LACTIC-ACID BACTERIA; IN-VITRO; SAFETY; BIFIDOBACTERIA; STRAIN;

D O I：

10.1155/2013/254239

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genotoxicity assessment is carried out on freeze dried powder of cultured probiotics containing Lactobacillus rhamnosus LCR177, Bifidobacterium adolescentis BA286, and Pediococcus acidilactici PA318. Ames tests, in vitro mammalian chromosome aberration assay, and micronucleus tests in mouse peripheral blood are performed. For 5 strains of Salmonella Typhimurium, the Ames tests show no increased reverse mutation upon exposure to the test substance. In CHO cells, the frequency of chromosome aberration does not increase in responding to the treatment of probiotics. Likewise, the frequency of micronucleated reticulocytes in probiotics-fed mice is indistinguishable from that in the negative control group. Taken together, the toxicity assessment studies suggest that the multispecies probiotic mixture does not have mutagenic effects on various organisms.

引用

页数：7