Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities

被引:65
|
作者
Ainsworth, TD [1 ]
Fine, M
Blackall, LL
Hoegh-Guldberg, O
机构
[1] Univ Queensland, Ctr Marine Studies, St Lucia, Qld 4072, Australia
[2] Univ Haifa, Leon Recanati Inst Maritime Studies, IL-31905 Haifa, Israel
[3] Univ Queensland, Adv Wastewater Management Ctr, Brisbane, Qld 4072, Australia
关键词
D O I
10.1128/AEM.72.4.3016-3020.2006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.
引用
收藏
页码:3016 / 3020
页数:5
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