Production of uracil from methane by a newly isolated Methylomonas sp SW1

被引:2
|
作者
Kim, Sangwoo [1 ]
Lee, Wangjun [1 ]
Song, Insu [2 ]
Kwon, Yuhyun [1 ]
Yun, Seokhun [3 ]
Park, Soohyun [1 ]
Cho, Sukhyeong [1 ]
Oh, Byung-Keun [1 ]
Bin Oh, Han [2 ]
Lee, Jinwon [1 ]
机构
[1] Sogang Univ, Dept Chem & Biomol Engn, 35 Baekbeom Ro, Seoul 04107, South Korea
[2] Sogang Univ, Dept Chem, 35 Baekbeom Ro, Seoul 04107, South Korea
[3] CJ CheilJedang, Biotechnol Res Inst, 55 Gwanggyo Ro 42 Gil, Suwon 16495, South Korea
基金
新加坡国家研究基金会;
关键词
C-1; compound; Isolation; Methane; Methylomonas sp; Uracil; METHYLOCOCCUS-CAPSULATUS BATH; METHANOTROPHIC BACTERIUM; MONOOXYGENASE; BIOCONVERSION; COPPER; GAS;
D O I
10.1016/j.jbiotec.2016.10.019
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu2+ concentration and temperature were investigated and optimized. As a result, Methylomonas sp. SW1 produced uracil from methane as a sole carbon source with a titer of 2.1 mg/L in 84 h without genetic engineering under the optimized condition. The results in this study demonstrate the feasibility of using Methylomonas sp. SW1 for the production of uracil from methane. This is the first report of uracil production from gas feedstock by methanotrophic bacteria. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:43 / 47
页数:5
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