A spectrofluorimetric method to determine acrivastine is proposed and applied to its determination in human urine and pharmaceuticals. The fluorimetric method allows the determination of 58-2000 ng ml(-1) of acrivastine in aqueous solutions containing acetic acid-sodium acetate buffer (pH 6.5) with lambda(exc) = 230 nm and lambda(em)=380 nm. (C) 2002 Elsevier Science B.V. All rights reserved.