Development of Peptide Nucleic Acid Probes for Detection of the HER2 Oncogene

被引：14

作者：

Metaferia, Belhu ^{[1
]}

Wei, Jun S. ^{[1
]}

Song, Young K. ^{[1
]}

Evangelista, Jennifer ^{[3
]}

Aschenbach, Konrad ^{[3
]}

Johansson, Peter ^{[1
]}

Wen, Xinyu ^{[1
,2
]}

Chen, Qingrong ^{[1
]}

Lee, Albert ^{[1
]}

Hempel, Heidi ^{[1
]}

Gheeya, Jinesh S. ^{[1
]}

Getty, Stephanie ^{[4
]}

Gomez, Romel ^{[3
]}

Khan, Javed ^{[1
]}

机构：

[1] NCI, Pediat Oncol Branch, NIH, Bethesda, MD 20892 USA

[2] NCI, Adv Biomed Comp Ctr, SAIC Frederick Inc, Frederick, MD 21701 USA

[3] Univ Maryland, Dept Elect & Comp Engn, College Pk, MD 20742 USA

[4] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA

来源：

PLOS ONE | 2013年 / 8卷 / 04期

基金：

美国国家卫生研究院;

关键词：

HYBRIDIZATION; DNA; PNA; IMMOBILIZATION; STABILITY; PNA/DNA;

D O I：

10.1371/journal.pone.0058870

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA. We have synthesized 15/16-mer PNA probes to detect the HER2 mRNA. The performance of these probes to detect the HER2 target was evaluated by fluorescence imaging and fluorescence bead assays. The PNA probes have sufficiently discriminated between the wild type HER2 target and the mutant target with single base mismatches. Furthermore, the probes exhibited excellent linear concentration dependence between 0.4 to 400 fmol for the target gene. The results demonstrate potential application of PNAs as diagnostic probes with high specificity for quantitative measurements of amplifications or over-expressions of oncogenes.

引用

页数：7

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