Cloning and characterization of a cDNA encoding phytoene synthase (PSY) in tea

被引：0
|
作者：
Borthakur, D. [1 ]
Du, Y. Y. [1 ]
Chen, H. [1 ]
Lu, J. L. [1 ]
Lin, C. [1 ]
Dong, J. J. [1 ]
Ye, J. H. [1 ]
Zheng, X. Q. [1 ]
Liang, Y. R. [1 ]
机构：
[1] Zhejiang Univ, Tea Res Inst, Hangzhou 310029, Peoples R China
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2008年 / 7卷 / 20期
基金：
美国国家科学基金会;
关键词：
Camellia sinensis; carotenoids; phytoene synthase; gene cloning; sequencing; phylogenetic tree;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Carotenoids are an important group of precursors of volatile flavour compounds in tea and phytoene synthase (PSY) is a key enzyme during the biosynthesis pathway of carotenoids. A cDNA encoding PSY in tea shoot [Camellia sinensis (L.) O. Kuntze] was cloned and sequenced in the present study. The obtained tea PSY cDNA was 1296 bp with an open reading frame of 987 bp. The predicted protein displayed a sequence of 329 amino acids. The phylogenetic tree showed that the tea PSY was clustered closely to the PSY of Adonis palaestina, with an overall amino acid identity of 90%.
引用
收藏
页码：3577 / 3581
页数：5
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