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Studies into the phenolic patterns of different tissues of pineapple (Ananas comosus [L.] Merr.) infructescence by HPLC-DAD-ESI-MS n and GC-MS analysis
被引:66
|作者:
Steingass, Christof B.
[1
]
Glock, Mona P.
[1
]
Schweiggert, Ralf M.
[1
]
Carle, Reinhold
[1
,2
]
机构:
[1] Univ Hohenheim, Inst Food Sci & Biotechnol, Chair Plant Foodstuff Technol & Anal, D-70599 Stuttgart, Germany
[2] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah 21589, Saudi Arabia
关键词:
Glycosides;
Phenolic acid esters;
Depsides;
Glutathione conjugates;
Spermidine derivatives;
PERFORMANCE LIQUID-CHROMATOGRAPHY;
HYDROXYCINNAMIC ACID-ESTERS;
SOLID-PHASE MICROEXTRACTION;
DEPENDENT METABOLIC-CHANGES;
TANDEM MASS-SPECTROMETRY;
TRIMETHYLSILYL DERIVATIVES;
ENZYMATIC-SYNTHESIS;
AROMA COMPOUNDS;
GLUCARIC ACID;
2-S-GLUTATHIONYLCAFTARIC ACID;
D O I:
10.1007/s00216-015-8811-2
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
In a comprehensive study, more than 60 phenolic compounds were detected in methanolic extracts from different tissues of pineapple infructescence by high-performance liquid chromatography with diode array detection and electrospray ionisation multiple-stage mass spectrometry (HPLC-DAD-ESI-MS (n) ) as well as by gas chromatography-mass spectrometry (GC-MS). The analytical workflow combining both methods revealed numerous compounds assigned for the first time as pineapple constituents by their mass fragmentations. Pineapple crown tissue was characterised by depsides of p-coumaric and ferulic acid. In contrast, major phenolic compounds in pineapple pulp extracts were assigned to diverse S-p-coumaryl, S-coniferyl and S-sinapyl derivatives of glutathione, N-l-gamma-glutamyl-l-cysteine and l-cysteine, which were also identified in the peel. The latter was additionally characterised by elevated concentrations of p-coumaric, ferulic and caffeic acid depsides and glycerides, respectively. Two peel-specific cyanidin hexosides were found. Elevated concentrations of isomeric N,N'-diferuloylspermidines may be a useful tool for the detection of fraudulent peel usage for pineapple juice production. Mass fragmentation pathways of characteristic pineapple constituents are proposed, and their putative biological functions are discussed.
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页码:6463 / 6479
页数:17
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