Persistence of mixed staphylococci assemblages following disinfection of hospital room surfaces

被引:19
|
作者
Sigler, V. [1 ]
Hensley, S. [2 ]
机构
[1] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA
[2] Univ Toledo, Infect Control Dept, Med Ctr, Toledo, OH 43606 USA
基金
美国国家科学基金会;
关键词
DGGE; Disinfection; Hospital infection; Staphylococcus; AUREUS; INFECTIONS; PCR;
D O I
10.1016/j.jhin.2012.12.009
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
The distribution of staphylococcal assemblages on surfaces in hospital rooms was assessed before and after daily disinfection with quaternary ammonia products. DNA was extracted from enrichment cultures of bacteria, which were swabbed from each of nine surface types, and subjected to analysis by staphylococci-specific, denaturing gradient gel electrophoresis. A genetic marker for Staphylococcus epidermidis/kloosii was detected on all surface types before and after cleaning, whereas markers for Staphylococcus aureus and Staphylococcus lugdunensis were detected on five surface types. Overall, genetic makers for several staphylococci known to colonize and infect humans remained ubiquitous in each room following daily disinfection practices. (C) 2013 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:253 / 256
页数:4
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