Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori

被引：1
|
作者：
Elliott, Paul R. [1 ]
Evans, Daniel [1 ]
Greenwood, Jacqueline A. [1 ]
Moody, Peter C. E. [1 ]
机构：
[1] Univ Leicester, Dept Biochem, Henry Wellcome Labs Struct Biol, Leicester LE1 9HN, Leics, England
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷
关键词：
D O I：
10.1107/S1744309108020320
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The classical glycolytic pathway contains an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, with NADP-dependent forms reserved for photosynthetic organisms and archaea. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori is reported; crystals of the protein were grown both in the presence and the absence of NADP.
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收藏
页码：723 / 726
页数：4
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