Female zebrafish (Danio rerio) are more vulnerable than males to microcystin-LR exposure, without exhibiting estrogenic effects

被引:63
|
作者
Qiao, Qin [1 ]
Liu, Wanjing [1 ]
Wu, Kang [1 ]
Song, Ting [1 ]
Hu, Jun [1 ]
Huang, Xiao [1 ]
Wen, Jing [1 ]
Chen, Liang [1 ]
Zhang, Xuezhen [1 ]
机构
[1] Huazhong Agr Univ, Coll Fisheries, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
基金
美国国家科学基金会;
关键词
Reproduction; Fish; Cyanobacterial blooms; Vitellogenin; Apoptosis; TERM REPRODUCTION ASSAY; FISH ORYZIAS-LATIPES; FATHEAD MINNOW; FRESH-WATER; IN-VITRO; CYANOBACTERIAL TOXIN; TISSUE DISTRIBUTIONS; SEASONAL DYNAMICS; SUBTROPICAL CHINA; RAT HEPATOCYTES;
D O I
10.1016/j.aquatox.2013.07.002
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Microcystins (MCs) released during cyanobacterial blooms exert varied toxicity on fish. Up to now, the reproductive toxicity of MCs on fish has rarely been reported. The present study investigated the reproductive toxicity of microcystin-LR (MC-LR) on male and female zebrafish (Danio rerio) by subchronic immersion in 1, 5,20 mu g/L for 30d. After MC-LR exposure, the hatchability and the 17 beta-estradiol (E2) concentration in gonads significantly decreased in the 20 mu g/L group. In the 5 and 20 mu g/L groups, the whole body vitellogenin (VTG) levels significantly increased in females, while considerably decreased in males. The VTG1 transcriptional level significantly reduced in the liver of both female and male treated fish. Marked histological lesions were observed in the livers, ovaries and testes in MC-LR treated fish. Apoptotic rate in the ovaries significantly increased. Significant down-regulation of Bcl-2 transcriptional level was found in the gonads of all MC-LR treated fish, while marked up-regulation of Bax transcription level was determined in the 20 mu g/L female treatment group, but a significant down-regulation in males. Although the transcriptional level of caspase-3 dropped in ovaries of the 5 and 20 mu g/L treatment groups, the significant increase of caspase-3 activation levels in the ovaries and testes were detected. The present findings indicate that MC-LR exposure exerts diverse reproductive toxicity in zebrafish with females exhibiting more sensitivity than males. The present study also confirmed for the first time that MC-LR does not cause any estrogenic effects in adult zebrafish. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:272 / 282
页数:11
相关论文
共 45 条
  • [31] Microcystin-LR immersion caused sequential endocrine disruption and growth inhibition in zebrafish (Danio rerio) from fertilization to sexual differentiation completion
    He, Ya
    Hou, Jie
    Qiu, Yuming
    Ouyang, Kang
    Li, Dapeng
    Li, Li
    TOXICOLOGY, 2023, 494
  • [32] Effects of enteric bacterial and cyanobacterial lipopolysaccharides, and of microcystin-LR, on glutathione S-transferase activities in zebra fish (Danio rerio)
    Best, JH
    Pflugmacher, S
    Wiegand, C
    Eddy, FB
    Metcalf, JS
    Codd, GA
    AQUATIC TOXICOLOGY, 2002, 60 (3-4) : 223 - 231
  • [33] Developmental estrogenic exposure in zebrafish (Danio rerio):: I.: Effects on sex ratio and breeding success
    Hill, RL
    Janz, DM
    AQUATIC TOXICOLOGY, 2003, 63 (04) : 417 - 429
  • [34] Female zebrafish are more affected than males under polystyrene microplastics exposure
    Wu, Di
    Carter, Laura
    Kay, Paul
    Holden, Joseph
    Yin, Ying
    Guo, Hongyan
    JOURNAL OF HAZARDOUS MATERIALS, 2025, 482
  • [35] Differential behavioral effects of ethanol pre-exposure in male and female zebrafish (Danio rerio)
    Clayman, Carly L.
    Malloy, Elizabeth J.
    Kearns, David N.
    Connaughton, Victoria P.
    BEHAVIOURAL BRAIN RESEARCH, 2017, 335 : 174 - 184
  • [36] Persistent Exposure to Environmental Levels of Microcystin-LR Disturbs Cortisol Production via Hypothalamic-Pituitary-Interrenal (HPI) Axis and Subsequently Liver Glucose Metabolism in Adult Male Zebrafish (Danio rerio)
    Wang, Lingkai
    Lin, Wang
    Zha, Qingji
    Guo, Honghui
    Zhang, Dandan
    Yang, Liping
    Li, Li
    Li, Dapeng
    Tang, Rong
    TOXINS, 2020, 12 (05)
  • [37] Transgenerational effects of extracts containing Microcystin-LR exposure on reproductive toxicity and offspring growth inhibition in a model organism zebrafish
    Yang, Qing
    Huang, Lanlan
    Yang, Ning
    Cui, Hongyang
    Zhao, Yanbin
    Li, Zipeng
    Tong, Yindong
    AQUATIC TOXICOLOGY, 2024, 268
  • [38] Microcystin-LR promotes zebrafish (Danio rerio) oocyte (in vivo) maturation by activating ERK1/2-MPF signaling pathways, and cAMP is involved in this process
    Zhan, Chunhua
    Zhang, Feng
    Liu, Wanjing
    Zhang, Xuezhen
    ENVIRONMENTAL POLLUTION, 2020, 259 (259)
  • [39] Effects of Maternal Cadmium Exposure on Female Reproductive Functions, Gamete Quality, and Offspring Development in Zebrafish (Danio rerio)
    Su Mei Wu
    Pei Ju Tsai
    Ming Yi Chou
    Wen-Der Wang
    Archives of Environmental Contamination and Toxicology, 2013, 65 : 521 - 536
  • [40] Effects of Maternal Cadmium Exposure on Female Reproductive Functions, Gamete Quality, and Offspring Development in Zebrafish (Danio rerio)
    Wu, Su Mei
    Tsai, Pei Ju
    Chou, Ming Yi
    Wang, Wen-Der
    ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2013, 65 (03) : 521 - 536