Characterization of immobilized laccase from Lentinula edodes and its use in olive-mill wastewater treatment

被引:137
|
作者
D'Annibale, A [1 ]
Stazi, SR [1 ]
Vinciguerra, V [1 ]
Di Mattia, E [1 ]
Sermanni, GG [1 ]
机构
[1] Univ Tuscia, Dept Agrobiol & Agrochem, I-01100 Viterbo, Italy
关键词
Lentinula edodes; laccase; immobilization; olive-mill wastewater; phenolic effluents; stabilization;
D O I
10.1016/S0032-9592(98)00144-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study is mainly concerned with the characterization of an immobilized laccase from the white-rot fungus Lentinula edodes for use in wastewater treatment. The enzyme was immobilized on chitosan by adsorption and subsequent cross-linking with glutaraldehyde. The immobilized laccase displayed a lower specific activity and a lower substrate affinity than the free enzyme. Nevertheless, the immobilized catalyst exhibited an appreciable catalytic capability (520 units g(-1) support) along with remarkably improved stability properties to various parameters, such as temperature, pH and storage time. The treatment of olive mill wastewater with immobilized laccase led to a partial decolorization as well as to significant abatements in its content in polyphenols, ortho-diphenols combined with a decreased toxicity of the effluent. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:697 / 706
页数:10
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