Immunogenicity and protective efficacy of an EB66® cell culture-derived duck Tembusu virus vaccine

The avian EB66 (R) cell line, derived from duck embryonic stem cells, has been widely used for producing human and animal therapeutic proteins and vaccines. In current study we evaluated the potential use of EB66 (R) cell line in a cell culture-derived duck Tembusu virus (DTMUV) vaccine development. After optimizing the growth conditions of DTMUV HB strain in EB66 (R) cells, we successfully generated three batches of viruses with ELD50 titres of 10(5.9)/0.1 ml, 10(5.3)/0.1 ml and 10(5.5)/0.1 ml, respectively, for using in the preparation of inactivated vaccines. The immunogenicity and protective efficacy of these EB66 (R) cells-derived inactivated vaccines were examined in ducks. Results indicated that all three batches of vaccines induced haemagglutination-inhibition (HI) antibody response in immunized birds at 2 weeks after a single immunization. Immunized ducks and ducklings were protected against a virulent challenge at 4 weeks after a booster immunization. The duration of immunity was for 3-4 months after a booster immunization. These results demonstrated the feasibility of using EB66 (R) cell line to grow up DTMUV for vaccine preparation.