Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation

被引:96
|
作者
Winchell, Jonas M. [1 ]
Thurman, Kathleen A. [1 ]
Mitchell, Stephanie L. [1 ]
Thacker, W. Lanier [1 ]
Fields, Barry S. [1 ]
机构
[1] Ctr Dis Control & Prevent, Resp Dis Branch, Atlanta, GA 30333 USA
关键词
D O I
10.1128/JCM.00440-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.
引用
收藏
页码:3116 / 3118
页数:3
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