Cellular internalization of PCL20-b-PEO44 block copolymer micelles

被引：101
|
作者：
Allen, C
Yu, YS
Eisenberg, A
Maysinger, D
机构：
[1] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada
[2] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1421卷 / 01期
基金：
加拿大自然科学与工程研究理事会;
关键词：
block copolymer micelles; endocytosis; drug delivery;
D O I：
10.1016/S0005-2736(99)00108-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cellular internalization of polycaprolactone-b-poly(ethylene oxide) (PCL20-b-PEO44) copolymer micelles were investigated in PC12 cells cultures. The micelles were found to be internalized into PC12 cells when followed over the 4-h incubation period. Also, the internalization process was found to fulfill the basic criteria for endocytotic uptake in that it was time, temperature, pH and energy dependent. In addition, the use of other pharmacological manipulations (hypertonic treatment, Brefeldin A) provide further evidence that the mode of cellular internalization is in fact endocytotic. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：32 / 38
页数：7
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