Micropropagation of an important Medicinal Plant Chlorophytum borivilianum

被引:0
|
作者
Haque, R. [1 ]
Saha, S. [1 ]
Bera, T. [1 ]
机构
[1] Jadavpur Univ, Div Plant Biotechnol, Dept Pharmaceut Technol, Kolkata 700032, WB, India
来源
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2009年 / 4卷 / 04期
关键词
Chlorophytum borrivillianum; Medicinal herb; Shoot culture; Callus culture; Michropropagation; Plant Regeneration; RAPID CLONAL PROPAGATION; VITRO; MULTIPLICATION; SUPERBA;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An efficient and large scale in vitro clonal propagation of valuable medicinal plant by enhanced shoot crown explant proliferation has been developed by us. Crown explants from Chlorophytum borivilianum initially treated with Bavistin 0.1% and 0.1% Endosulphan and 0.05% Fluconazole were cultured in solidified modified Murashige and skoog (MS) media, each with different hormonal combination for the establishment of cultures, multiplication and rooting of plants. Several plant growth regulators BAP(6 Benzyl aminopurine), Kinetine (Kn), IAA (Indole Acetic acid), NAA(alpha-Naphthylacetic acid) alone or in combination were tested for their capacity to induce shoot from shoot crown explants. For establishment of the shoot crown explants 2 mg/l Kinetin and I mg/l IBA were necessary. Average 73 shoots were generated when established cultures were transferred to modified MS medium containing 3 mg/l Kinetine and 2 mg/l BAP. Rooting was initiated in the half strength modified MS basal medium containing 30 gm/l sucrose(w/v) supplemented with 1 mg/l IBA (Indole buteyric acid). Strong rooted plantlets obtained after 3-4 weeks growth were transferred to soil-sand vermicompost (1:1:1 v/v) with more than 95% success in hardening. High multiplication efficiency, phenotypic stability and yield ensure the efficacy of the protocol developed for the production of medicinal herb safed musli.
引用
收藏
页码:5 / 11
页数:7
相关论文
共 50 条
  • [31] Meta-topolin mediated improved micropropagation, phytochemical profiling, and assessment of genetic fidelity in Chlorophytum borivilianum Sant. et Fernand., an endangered medicinal herb
    Karwasra, Rohtas Kumar
    Nazir, Romaan
    Pandey, Devendra Kumar
    INDUSTRIAL CROPS AND PRODUCTS, 2024, 214
  • [32] Steroidal saponins from the roots of Chlorophytum borivilianum
    Acharya, D.
    Mitaine-Offer, A. C.
    Kaushik, N.
    Miyamoto, T.
    Paululat, T.
    Lacaille-Dubois, M. A.
    PLANTA MEDICA, 2008, 74 (09) : 1033 - 1033
  • [33] Immunomodulatory Polysaccharide from Chlorophytum borivilianum Roots
    Thakur, Mayank
    Connellan, Paul
    Deseo, Myrna A.
    Morris, Carol
    Dixit, Vinod K.
    EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2011, 2011 : 1 - 7
  • [34] Scaling-up production and field performance of micropropagated medicinal herb ‘Safed Musli’ (Chlorophytum borivilianum)
    Ashish Dave
    Geeta Bilochi
    Sunil D. Purohit
    In Vitro Cellular & Developmental Biology - Plant, 2003, 39 : 419 - 424
  • [35] Studies on storage behaviour of Chlorophytum borivilianum genotypes
    Chandra, Ram
    Kumar, Dinesh
    INDIAN JOURNAL OF HORTICULTURE, 2007, 64 (02) : 199 - 201
  • [36] Isolation and characterization of phytoconstituents from Chlorophytum borivilianum
    Deore, Sharada L.
    Khadabadi, Somshekhar S.
    PHARMACOGNOSY RESEARCH, 2010, 2 (06): : 343 - 349
  • [37] Furostanol glycosides from the roots of Chlorophytum borivilianum
    Acharya, D.
    Mitaine-Offer, A. C.
    Kaushik, N.
    Miyamoto, T.
    Lacaille-Dubois, M. A.
    PLANTA MEDICA, 2007, 73 (09) : 958 - 958
  • [38] Physiological and molecular characterization of in vitro cultures of an endemic medicinal herb, Chlorophytum borivilianum, under abiotic stress
    Debnath, Mousumi
    Pandey, Mukeshwar
    Chikara, Surendra K.
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (38): : 7356 - 7366
  • [39] Scaling-up production and field performance of micropropagated medicinal herb 'safed musli' (Chlorophytum borivilianum)
    Dave, A
    Bilochi, G
    Purohit, SD
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2003, 39 (04) : 419 - 424
  • [40] Establishment of an efficient protocol for micropropagation of stem explants of Tylophora indica, an important medicinal plant
    Kaur, Harmanjit
    Anand, Manju
    Goyal, Dinesh
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (36): : 6928 - 6932