High engraftment capacity of frozen ready-to-use human fecal microbiota transplants assessed in germ-free mice

被引：6

作者：

Berland, Magali ^{[1
]}

Cadiou, Julie ^{[2
]}

Levenez, Florence ^{[1
]}

Galleron, Nathalie ^{[1
]}

Quinquis, Benoit ^{[1
]}

Thirion, Florence ^{[1
]}

Gauthier, Franck ^{[1
]}

Le Chatelier, Emmanuelle ^{[1
]}

Onate, Florian Plaza ^{[1
]}

Schwintner, Carole ^{[3
]}

Rabot, Sylvie ^{[2
]}

Lepage, Patricia ^{[2
]}

Ehrlich, Dusko ^{[1
]}

Dore, Joel ^{[1
,2
]}

Juste, Catherine ^{[1
,2
]}

机构：

[1] Univ Paris Saclay, INRAE, MGP, F-78350 Jouy En Josas, France

[2] Univ Paris Saclay, INRAE, AgroParisTech, Micalis Inst, F-78350 Jouy En Josas, France

[3] MaaT Pharma, Pharmaceut Dev, F-69007 Lyon, France

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

CLOSTRIDIUM-DIFFICILE INFECTION; BUTYRATE-PRODUCING BACTERIA; RECURRENT; VIABILITY; OBESITY; SAMPLES; STOOL;

D O I：

10.1038/s41598-021-83638-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The number of indications for fecal microbiota transplantation is expected to rise, thus increasing the needs for production of readily available frozen or freeze-dried transplants. Using shotgun metagenomics, we investigated the capacity of two novel human fecal microbiota transplants prepared in maltodextrin-trehalose solutions (abbreviated MD and TR for maltodextrin:trehalose, 3:1, w/w, and trehalose:maltodextrin 3:1, w/w, respectively), to colonize a germ-free born mouse model. Gavage with frozen-thawed MD or TR suspensions gave the taxonomic profiles of mouse feces that best resembled those obtained with the fresh inoculum (Spearman correlations based on relative abundances of metagenomic species around 0.80 and 0.75 for MD and TR respectively), while engraftment capacity of defrosted NaCl transplants most diverged (Spearman correlations around 0.63). Engraftment of members of the family Lachnospiraceae and Ruminoccocaceae was the most challenging in all groups of mice, being improved with MD and TR transplants compared to NaCl, but still lower than with the fresh preparation. Improvement of engraftment of this important group in maintaining health represents a challenge that could benefit from further research on fecal microbiota transplant manufacturing.

引用

页数：10

共 42 条

[41] Colonization of Germ-Free Mice by Human Gut Microbiota Alters Colonic Morphology and Increases Tph1 Expression Likely Contributing to Altered Colonic Contractility Following Colonization
Reigstad, Christopher S.
Salmonson, Charles E.
Linden, David R.
Szurszewski, Joseph H.
Sonnenburg, Justin L.
Farrugia, Gianrico
Kashyap, Purna C.
GASTROENTEROLOGY, 2014, 146 (05) : S7 - S8
[42] Functional Interactions between Gut Microbiota Transplantation, Quercetin, and High-Fat Diet Determine Non-Alcoholic Fatty Liver Disease Development in Germ-Free Mice
Porras, David
Nistal, Esther
Martinez-Florez, Susana
Luis Olcoz, Jose
Jover, Ramiro
Jorquera, Francisco
Gonzalez-Gallego, Javier
Victoria Garcia-Mediavilla, Maria
Sanchez-Campos, Sonia
MOLECULAR NUTRITION & FOOD RESEARCH, 2019, 63 (08)

← 1 2 3 4 5 →