Nodular lymphocyte predominant Hodgkin lymphoma - An immunophenotypic reappraisal based on a single-institution experience

In our experience, certain commonly cited immunophenotypic features of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) are not encountered in day-to-day practice. We reviewed 60 cases of NLPHL (18 women, 42 men; median age, 34 years) to discern immunophenotypic features from a large, single-institution cohort. All cases contained lymphocytic and histiocytic (L&H) cells. These cells expressed CD20 in 98% (59160), CD79a (usually faint) in 87% (27131), CD30 in 7% (4159), epithelial membrane antigen in 21% (12156), bcl-2 in 5% (2141), and bcl-6 in 83% (30136) of cases. CD10 was negative in all 36 cases studied; 100% of cases (55155) demonstrated CD3+ rosettes. Although CD57+ T cells were common within the background infiltrate, CD57+ rosettes were seen in only 48% of cases (15131) and were rare when encountered. Based on these patterns, we conclude that bcl-2 and bcl-6 may be useful additions to the immunophenotypic analysis of NLPHL, but that the diagnostic usefulness of epithelial membrane antigen and CD57 rosettes may have been overemphasized in previous reports.