A simple "mix-and-detection" method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions

被引：41

作者：

Ma, Fei ^{[1
]}

Wei, Shu-hua ^{[1
]}

Leng, Junhong ^{[2
]}

Tang, Bo ^{[1
]}

Zhang, Chun-yang ^{[1
]}

机构：

[1] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Shandong, Peoples R China

[2] Jinan Matern & Child Care Hosp, Jinan 250000, Shandong, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 20期

基金：

中国国家自然科学基金;

关键词：

ROLLING-CIRCLE AMPLIFICATION; TARGET RECYCLING AMPLIFICATION; REAL-TIME; SIGNAL AMPLIFICATION; INTRACELLULAR TELOMERASE; FLUORESCENCE DETECTION; GOLD NANOPARTICLES; MOLECULAR BEACON; NUCLEIC-ACIDS; ASSAY;

D O I：

10.1039/c8cc00093j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We develop a simple mix-and-detection method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions. This method enables one-step and one-tube detection of telomerase with a detection limit of 3 cells, and it can be further applied for the screening of telomerase inhibitors and the discrimination of cancer cells from normal cells.

引用

页码：2483 / 2486

页数：4

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