USING DIFFERENT ELECTROPHORETIC METHOD FOR THE DETERMINATION OF THE AFFINITY CONSTANTS OF SALICYLIC ACID AND BSA

The capillary electrophoresis fontal analysis (CE-FA), Hummel Dreyer methods (HD) and affinity capillary electrophoresis (ACE) were used to study the affinities between salicylic acid (SA) and bovine serum albumin (BSA). The binding constant (Ka) was measured by all these methods. The Ka values obtained from CE-FA (5.66 +/- 0.22 x 10(3)M(-1)), HD methods with internal (2.83 +/- 0.23 x 10(3)M(-1)) and external (2.28 +/- 0.12 x 10(3)M(-1)) calibration are in agreement. The comparison of results and other conditions shows the best measurement methods are CE-FA and HD with external calibration.