Novel polyethylene glycol/levan aqueous two-phase system for protein partitioning

被引：9
|
作者：
Chung, BH [1 ]
Kim, WK [1 ]
Song, KB [1 ]
Kim, CH [1 ]
Rhee, SK [1 ]
机构：
[1] KOREA INST BIOSCI & BIOTECHNOL,MICROBIAL METAB ENGN RES UNIT,TAEJON 305600,SOUTH KOREA
来源：
BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 05期
关键词：
D O I：
10.1023/A:1018475529976
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel aqueous two-phase system was made by mixing PEG (polyethylene glycol) and levan. The partitioning experiments were performed with six model proteins: horse heart cytochrome c, horse hemoglobin, horse heart myoglobin, hen egg albumin, bovine serum albumin and hen egg lysozyme. The addition of NaCl increased the partition coefficient of lysozyme but decreased that of albumin. The partition coefficients were sensitive to pH, increasing in the pH range of 6 to 8 for five of the proteins tested and decreasing slightly for lysozyme.
引用
收藏
页码：327 / 329
页数：3
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