Rare Plant of Central Yakutia Polygala sibirica L.: Phytochemical Profile and In Vitro Morphogenic Culture

Polygala sibirica L. is a rare and endangered plant species that is listed in the Red Book of the Republic of Sakha (Yakutia) under category "3 c" as a species with a narrow ecological limitation to limestone rock outcrops. For the first time, the phytochemical profile of the above-ground phytomass of P. sibirica growing in Central Yakutia was studied, and in vitro culture induction was performed. Using HPLC-MS/MS and tandem mass spectrometry methods, 74 compounds were allegedly identified in methanol extracts of the above-ground phytomass of the P. sibirica, of which 40 compounds were identified for the first time within the genus Polygala L., including 22 polyphenolic compounds: flavones (isoformononetin, syringetin, apigenin 7-O-glucoside, etc.), flavonols (herbacetin, myricetin, etc.), flavan-3-ols (afselequin, epicatechin, etc.), flavanones (hesperitin, eriocitrin), phenolic acid, and anthocyanins. A primary callus culture of P. sibirica was based on leaf explants of intact plants with the study of the dynamics of growth of raw and dried cell biomass. On the basis of callus tissue, indirect morphogenesis with abundant shoot formation is initiated. Thus, the basis has been laid for the subsequent propagation of the rare plant P. sibirica for the purpose of reintroduction in the conditions of the Yakut Botanical Garden.