Isolation and Characterization of the First Zobellviridae Family Bacteriophage Infecting Klebsiella pneumoniae

被引:5
|
作者
Gorodnichev, Roman B. [1 ]
Kornienko, Maria A. [1 ]
Malakhova, Maja V. [1 ]
Bespiatykh, Dmitry A. [1 ]
Manuvera, Valentin A. [1 ]
Selezneva, Oksana V. [1 ]
Veselovsky, Vladimir A. [1 ]
Bagrov, Dmitry V. [1 ,2 ]
Zaychikova, Marina V. [1 ]
Osnach, Veronika A. [3 ]
Shabalina, Anna V. [3 ]
Goloshchapov, Oleg V. [4 ]
Bespyatykh, Julia A. [1 ]
Dolgova, Anna S. [3 ]
Shitikov, Egor A. [1 ]
机构
[1] Fed Med Biol Agcy, Lopukhin Fed Res & Clin Ctr Phys Chem Med, Moscow 119435, Russia
[2] Lomonosov Moscow State Univ, Fac Biol, Dept Bioengn, Moscow 119234, Russia
[3] St Petersburg Pasteur Inst, Fed Serv Consumer Rights Protect & Human Well Bei, St Petersburg 197101, Russia
[4] Pavlov First St Petersburg State Med Univ, RM Gorbacheva Mem Inst Oncol Hematol & Transplant, St Petersburg 197022, Russia
关键词
Klebsiella pneumoniae; multidrug resistance; capsular type; capsule depolymerase; bacteriophage; Zobellviridae; TRANSFER-RNA GENES; COMPLETE SEQUENCE; RESISTANCE; PHI-RIO-1;
D O I
10.3390/ijms24044038
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In order to address the upcoming crisis in the treatment of Klebsiella pneumoniae infections, caused by an increasing proportion of resistant isolates, new approaches to antimicrobial therapy must be developed. One approach would be to use (bacterio)phages and/or phage derivatives for therapy. In this study, we present a description of the first K. pneumoniae phage from the Zobellviridae family. The vB_KpnP_Klyazma podovirus, which forms translucent halos around the plaques, was isolated from river water. The phage genome is composed of 82 open reading frames, which are divided into two clusters located on opposite strands. Phylogenetic analysis revealed that the phage belongs to the Zobellviridae family, although its identity with the closest member of this family was not higher than 5%. The bacteriophage demonstrated lytic activity against all (n = 11) K. pneumoniae strains with the KL20 capsule type, but only the host strain was lysed effectively. The receptor-binding protein of the phage was identified as a polysaccharide depolymerase with a pectate lyase domain. The recombinant depolymerase protein showed concentration-dependent activity against all strains with the KL20 capsule type. The ability of a recombinant depolymerase to cleave bacterial capsular polysaccharides regardless of a phage's ability to successfully infect a particular strain holds promise for the possibility of using depolymerases in antimicrobial therapy, even though they only make bacteria sensitive to environmental factors, rather than killing them directly.
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页数:14
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