Overexpression of Two MADS-Box Genes from Lagerstroemia speciosa Causes Early Flowering and Affects Floral Organ Development in Arabidopsis

被引:4
|
作者
Yang, Lichen [1 ,2 ,3 ,4 ,5 ,6 ]
Li, Zhuojiao [1 ,2 ,3 ,4 ,5 ,6 ]
Zheng, Tangchun [1 ,2 ,3 ,4 ,5 ,6 ]
Wang, Jia [1 ,2 ,3 ,4 ,5 ,6 ]
Cheng, Tangren [1 ,2 ,3 ,4 ,5 ,6 ]
Zhang, Qixiang [1 ,2 ,3 ,4 ,5 ,6 ]
机构
[1] Beijing Forestry Univ, Beijing Key Lab Ornamental Plants Germplasm Innova, Beijing 100083, Peoples R China
[2] Beijing Forestry Univ, Natl Engn Res Ctr Floriculture, Beijing 100083, Peoples R China
[3] Beijing Forestry Univ, Beijing Lab Urban & Rural Ecol Environm, Beijing 100083, Peoples R China
[4] Beijing Forestry Univ, Engn Res Ctr Landscape Environm, Minist Educ, Beijing 100083, Peoples R China
[5] Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamental, Minist Educ, Beijing 100083, Peoples R China
[6] Beijing Forestry Univ, Sch Landscape Architecture, Beijing 100083, Peoples R China
来源
AGRONOMY-BASEL | 2023年 / 13卷 / 04期
关键词
Lagerstroemia speciosa; MADS-box gene; LsAG2; LsDEF1; flowering time; flower development; ORTHOLOGOUS GENE; EXPRESSION;
D O I
10.3390/agronomy13040976
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Lagerstroemia speciosa is an important ornamental plant, and there is only one double-petaled variety, 'Yunshang', produced by natural mutation, in the whole genus of Lagerstroemia. The members of the MADS-box family play important roles in floral organ development. However, little is known about the biological function of the MADS-box gene in L. speciosa. In our study, two MADS-box genes (LsAG2 and LsDEF1) were isolated from L. speciosa, and their expression levels in different tissues and developmental stages were analyzed by RT-qPCR. Subcellular localization showed that LsAG2 and LsDEF1 are localized in the nucleus. The overexpression of LsAG2 and LsDEF1 in Arabidopsis thaliana caused transgenic plants to exhibit different phenotypes, such as floral organ aberrations, early flowering, and dwarf plants, and resulted in the up-regulation of endogenous genes related to flowering (i.e., AP1, PI, FLC, FUL, LFY, and FT). Our results provide a theoretical basis for the flowering time, flower development, and genetic improvement of double-petaled flowers in the genus of Lagerstroemia.
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页数:12
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