FREEZING OF EQUINE SEMEN IS INFLUENCED BY EXPOSURE TIME AND CONCENTRATION OF THE CRYOPROTECTANT GLYCEROL

被引：0

作者：

Fracaro, Pablo Luis ^{[1
]}

Corcini, Carine Dahl ^{[2
,3
]}

Gatti, Norton Luis Souza ^{[1
]}

Filho, Jorge Squeff ^{[1
]}

Acosta, Izani Bonel ^{[1
]}

Filho, Jorge Squeff ^{[1
]}

Hartwig, Felipe Pires ^{[1
]}

Curcio, Bruna Da Rosa ^{[1
]}

Varela Jr, Antonio Sergio ^{[2
,3
]}

机构：

[1] Univ Fed Pelotas, Fac Vet, Programa Posgrad Vet, Pelotas, RS, Brazil

[2] Univ Fed Rio Grande, Reprod Anim Comparada RAC, Inst Ciencias Biol, Rio Grande, RS, Brazil

[3] Univ Fed Pelotas, Fac Vet, ReproPel, Pelotas, RS, Brazil

来源：

CRYOLETTERS | 2023年 / 44卷 / 04期

关键词：

conservation; sperm; equine; motility; STALLION SEMEN; CRYOPRESERVATION; FROZEN;

D O I：

10.54680/fr23410110412

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

BACKGROUND: Glycerol is a cryoprotectant widely used in the freezing of mammalian species' semen, but no study has demonstrated its optimum concentration and the appropriate exposure time for equine species. OBJECTIVE: To demonstrate that the exposure time (15, 30, 45, 60, 75 and 90 min) versus concentration (2, 3, 4 and 5%) of the cryoprotectant glycerol influences the freezing success of equine semen. MATERIALS AND METHODS: The ejaculate of 12 stallions were frozen in different glycerol concentrations following different exposure times. The thawed sperm was evaluated for kinetic parameters using a Computer Assisted Semen Analysis (CASA) system and cell feature parameters were assessed by flow cytometry. RESULTS: Considering the total and progressive motility of the spermatozoa, we concluded that protocols using 5% glycerol for 15 and 30 min exposure, 4% glycerol for 45 min exposure and 3% glycerol for 90 min exposure generated the best results. CONCLUSION: We suggest the use of any of these protocols for a better cryopreservation of equine semen.

引用

页码：234 / 239

页数：6

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