One of a common commercial and culinary fruits from the Solanaceae family is the Cape goose-berry. Due to their enormous medicinal potential to treat a variety of ailments, the Physalis species have been employed traditionally. The Physalis species has a wide range of bioactivities based on the presence of secondary metabolites, including immunomodulatory, cytotoxic, anti-diabetic, antioxidant, anti-cholesterolemic, and antimicrobial. The plants gathered from various parts of Himachal Pradesh are subjected to meiotic exams in this study. The purpose of the current research was to study the ploidy levels of the aforementioned Physalis species using conventional chromosome counting techniques. More cytotypes, morphotypes, and chemotypes must be identi-fied in order to conduct future breeding experiments for getting a crop with high medicinal content and stress tol- erant variety. Therefore, morphological and meiotic analyses of three Indian Physalis species were performed. P. angulata L. (2n=4x= 48) demonstrates a tetraploid nature, whereas P. peruviana L. (2n=2x= 24) and P. longifolia Nutt. (2n=2x= 24) are diploid.