Comparison of two analytical methods for detecting Clostridium spores in milk

被引：7

作者：

Andrighetto, Christian ^{[1
]}

Spolaor, Dino

De Dea, Paola ^{[1
]}

Fioravanzo, Elisa ^{[1
]}

Brazzale, Piercristiano ^{[2
]}

De Battista, Matteo ^{[3
]}

Dolci, Elisa ^{[4
]}

Macca, Francesca ^{[5
]}

Sperotto, Davide ^{[3
]}

Lombardi, Angiolella

机构：

[1] Agenzia Veneta Innovaz Settore Primario, Via San Gaetano 74, I-36016 Thiene, VI, Italy

[2] Brazzale SPA, Via Pasubio 2, I-36010 Zane, VI, Italy

[3] Lattebusche SCA, Via Nazl 59, I-32030 Busche Di Cesiomaggiore, BL, Italy

[4] Ind Casearia Silvio Belladelli, Via Pasubio 49, I-37069 Villafranca Dasti, VR, Italy

[5] Latteria Soligo, Via 1 Settembre 32, I-31020 Soligo, TV, Italy

来源：

INTERNATIONAL DAIRY JOURNAL | 2023年 / 142卷

关键词：

Analytical method - Analytical performance - Genus bacillus - Milk sample - Raw milk;

D O I：

10.1016/j.idairyj.2023.105649

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A study involving analysis of raw milk samples naturally contaminated by Clostridium spores was organised and the analytical performance of AmpMedia 666 broth was evaluated in comparison with the conventional MPN method based on the use of reinforced clostridial medium (RCM) broth. The selec-tivity of RCM medium in detecting Clostridium after 2 days, 5 days and 7 days of incubation at 37 degrees C was 88%, 78% and 65%, respectively, while the selectivity of AmpMedia 666 was 96%. Most of the colonies not belonging to the genus Clostridium were assigned to the spore forming genera Bacillus, Virgibacillus and Rummeliibacillus.(c) 2023 Elsevier Ltd. All rights reserved.

引用

页数：4

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