Generation of a TRPM8 knockout hESC line (WAe009-A-A) derived from H9 using CRISPR/Cas9

被引：0
|
作者：
Bai, Jinmeng [1 ,2 ]
Zhang, Jianchao [1 ,2 ]
Fu, Wanrong [1 ,2 ]
Li, Shuang [1 ,2 ]
Tian, Xiaoxu [1 ,2 ]
Li, Xiaowei [1 ,2 ]
Zhao, Xiaoyan [1 ,2 ]
Dong, Jianzeng [1 ,2 ,3 ]
机构：
[1] Zhengzhou Univ, Dept Cardiol, Affiliated Hosp 1, Zhengzhou 450052, Peoples R China
[2] Henan Key Lab Hereditary Cardiovasc Dis, Zhengzhou 450052, Peoples R China
[3] Capital Med Univ, Beijing Anzhen Hosp, Natl Clin Res Ctr Cardiovasc Dis, Dept Cardiol, 2 Beijing Anzhen Rd, Beijing 100029, Peoples R China
来源：
STEM CELL RESEARCH | 2023年 / 67卷
关键词：
D O I：
10.1016/j.scr.2023.103040
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The transient receptor potential cation channel subfamily M member 8 (TRPM8) is a kind of non-selective cation channel which controls Ca2+ homeostasis. Mutations in TRPM8 were related to dry eye diseases (DED). Here we constructed a TRPM8 knockout cell line WAe009-A-A from the original embryonic stem cell line H9 using CRISPR/Cas9 technology, which maybe helpful for exploring the pathogenesis of DED. WAe009-A-A cells possess stem cell morphology and pluripotency as well as normal karyotype, and have the ability of differentiating into three germ layers in vitro.
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页数：5
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