SINGLE-CELLULAR DYNAMIC MECHANICAL ANALYSIS OF LIVE 3D ORGANOIDS UNDER LIGHT-SHEET FLUORESCENCE MICROSCOPY

被引：0
|
作者：
Kidambi, Venkatanathan [1 ]
Tomizawa, Yuji [1 ]
Surti, Manav [1 ]
Modarelli, Mitchell [1 ]
Hoshino, Kazunori [1 ]
机构：
[1] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA
来源：
2024 IEEE 37TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS | 2024年
基金：
美国国家科学基金会;
关键词：
Light sheet microscopy; dynamic mechanical analysis; organoids; mechanical characterization; MECHANOBIOLOGY;
D O I：
10.1109/MEMS58180.2024.10439442
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We have developed a novel microscopic analysis system that combines the functions of light-sheet fluorescence microscopy (LSM) and dynamic mechanical analysis (DMA). We have integrated the three uniquely designed components of (i) a MEMS dynamic compression device with a mu-force sensor, (ii) a high-speed 3D light-sheet scanner and an imager, and (iii) a customprogrammed image-based 3D modeling algorithm. Here, we demonstrate spatially-resolved mechanical characterization of viscoelastic materials under high-resolution 3D fluorescence microscopy for the first time.
引用
收藏
页码：330 / 333
页数：4
相关论文
共 50 条
  • [31] Tilted light-sheet microscopy with 3D point-spread functions
    Nature Methods, 2018, 15 (3) : 163 - 163
  • [32] Cell lines and clearing approaches: a single-cell level 3D light-sheet fluorescence microscopy dataset of multicellular spheroids
    Diosdi, Akos
    Hirling, Dominik
    Kovacs, Maria
    Toth, Timea
    Harmati, Maria
    Koos, Krisztian
    Buzas, Krisztina
    Piccinini, Filippo
    Horvath, Peter
    DATA IN BRIEF, 2021, 36
  • [33] Single-cell light-sheet fluorescence 3D images of tumour-stroma spheroid multicultures
    Akos Diosdi
    Filippo Piccinini
    Timea Boroczky
    Gabriella Dobra
    Gastone Castellani
    Krisztina Buzas
    Peter Horvath
    Maria Harmati
    Scientific Data, 12 (1)
  • [34] Bright fluorescent probe enables analyte responsive, 3D imaging of H2S in live zebrafish using light-sheet fluorescence microscopy
    Hammers, Matthew
    Pluth, Michael
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 251
  • [35] Development a flexible light-sheet fluorescence microscope for high-speed 3D imaging of calcium dynamics and 3D imaging of cellular microstructure
    Sparks, Hugh
    Dvinskikh, Liuba
    Firth, Jahn M.
    Francis, Alice J.
    Harding, Sian E.
    Paterson, Carl
    MacLeod, Ken T.
    Dunsby, Chris
    JOURNAL OF BIOPHOTONICS, 2020, 13 (06)
  • [36] Live imaging of Tribolium castaneum embryonic development using light-sheet–based fluorescence microscopy
    Frederic Strobl
    Alexander Schmitz
    Ernst H K Stelzer
    Nature Protocols, 2015, 10 : 1486 - 1507
  • [37] Fast 3D imaging of giant unilamellar vesicles using reflected light-sheet microscopy with single molecule sensitivity
    Jannasch, Anita
    Szilagyi, Sven A.
    Burmeister, Moritz
    Davis, Q. Tyrell
    Hermsdorf, Gero L.
    De, Suman
    Schaeffer, Erik
    JOURNAL OF MICROSCOPY, 2022, 285 (01) : 40 - 51
  • [38] Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy
    Held, Marie
    Santeramo, Ilaria
    Wilm, Bettina
    Murray, Patricia
    Levy, Raphael
    PLOS ONE, 2018, 13 (07):
  • [39] 3D print customized sample holders for live light sheet microscopy
    Jeandupeux, Emeric
    Lobjois, Valerie
    Ducommun, Bernard
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2015, 463 (04) : 1141 - 1143
  • [40] High-content screening of 3D cell culture models with light-sheet microscopy
    不详
    JOURNAL OF CELL SCIENCE, 2020, 133 (11)
12345