SINGLE-CELLULAR DYNAMIC MECHANICAL ANALYSIS OF LIVE 3D ORGANOIDS UNDER LIGHT-SHEET FLUORESCENCE MICROSCOPY

被引：0
|
作者：
Kidambi, Venkatanathan [1 ]
Tomizawa, Yuji [1 ]
Surti, Manav [1 ]
Modarelli, Mitchell [1 ]
Hoshino, Kazunori [1 ]
机构：
[1] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA
来源：
2024 IEEE 37TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS | 2024年
基金：
美国国家科学基金会;
关键词：
Light sheet microscopy; dynamic mechanical analysis; organoids; mechanical characterization; MECHANOBIOLOGY;
D O I：
10.1109/MEMS58180.2024.10439442
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We have developed a novel microscopic analysis system that combines the functions of light-sheet fluorescence microscopy (LSM) and dynamic mechanical analysis (DMA). We have integrated the three uniquely designed components of (i) a MEMS dynamic compression device with a mu-force sensor, (ii) a high-speed 3D light-sheet scanner and an imager, and (iii) a customprogrammed image-based 3D modeling algorithm. Here, we demonstrate spatially-resolved mechanical characterization of viscoelastic materials under high-resolution 3D fluorescence microscopy for the first time.
引用
收藏
页码：330 / 333
页数：4
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