In vitro regeneration of triploid from mature endosperm culture of Passiflora edulis "Mantianxing"

被引：1

作者：

Tang, Junrong ^{[1
,2
]}

Luo, Xi ^{[1
,2
]}

Zhu, Yajing ^{[1
,2
]}

Cai, Nianhui ^{[1
,2
]}

Chen, Lin ^{[1
,2
]}

Chen, Shi ^{[1
,2
]}

Xu, Yulan ^{[1
,2
]}

机构：

[1] Southwest Forestry Univ, Key Lab Natl Forestry & Grassland Adm Biodivers Co, Kunming, Peoples R China

[2] Southwest Forestry Univ, Coll Forestry, Kunming, Peoples R China

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2024年 / 88卷 / 04期

关键词：

Passiflora edulis "Mantianxing; endosperm; plant regeneration; triploid plants; ploidy identification; PLANT-REGENERATION; POLYPLOIDY; EFFICIENT; GROWTH; L;

D O I：

10.1093/bbb/zbae009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The regeneration of shoots from endosperm tissue is a highly effective method to obtain triploid plants. In this study, we elucidated the establishment of an in vitro regeneration system from endosperm culture for the production of Passiflora edulis "Mantianxing." The highest callus induction rate (83.33%) was obtained on the media supplemented with 1.0 mg/L TDZ. Meanwhile, the MS medium containing 1.0 mg/L 6-BA and 0.4 mg/L IBA gave the optimum 75% shoot bud induction. Chromosome analysis revealed that the chromosomal count of P. edulis "Mantianxing" regenerated from endosperm tissues was 27 (2n = 3x = 27), which indicated that shoots regenerated from endosperm tissues were triploids. Triploid P. edulis had more drought resistance than diploid plants. Our study provided a method for breeding of passion fruit by means of a stable and reproducible regeneration system from endosperm culture, leading to the generation of triploid plants. Graphical Abstract Triploid plants were obtained by endosperm culture of Passiflora edulis.

引用

页码：412 / 419

页数：8

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