Background: The prognostic and predictive role of stromal tumor-infiltrating lymphocytes (sTILs) is undetermined in pleomorphic invasive lobular cancer (pILC). The same applies for the expression of PD-1/PD-L1 in this rare breast cancer subtype. Here, we aimed to investigate the expression of sTILs and analyze the PD-L1 expression levels in pILC. Methods: Archival tissues from sixty-six patients with pILC were collected. The sTIL density was scored as a percentage of tumor area using the following cut-offs: 0%; <5%; 5-9%; and 10-50%. The PD-L1 expression was analyzed using IHC on formalin-fixed, paraffin-embedded tissue sections using SP142 and 22C3 antibodies. Results: A total of 82% of the sixty-six patients were hormone receptor positive and 8% of cases were triple negative (TN), while 10% showed human epidermal growth factor receptor 2 (HER2) amplification. sTILs (=1%) were present in 64% of the study population. Using the SP142 antibody, 36% of tumors demonstrated a positive PD-L1 score of =1%, and using the 22C3 antibody, 28% had a positive PD-L1 score of =1. There was no correlation between sTILs or PD-L1 expression and tumor size, tumor grade, nodal status, expression of estrogen receptor (ER), or amplification of HER2. Our data did not show any difference in survival between the three molecular subtypes of pILC with respect to sTILs and PD-L1 expression. Conclusion: This study shows that pILCs show some degree of sTILs and PD-L1 expression; however, this was not associated with a survival improvement. Additional large trials are needed to understand immune infiltration in lobular cancer, especially in the pleomorphic subtype.
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Tokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
Hanamura, Toru
Kitano, Shigehisa
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Canc Inst Hosp JFCR, Ctr Adv Med Dev, Div Canc Immunotherapy Dev, 3-8-31 Ariake, Tokyo 1358550, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
Kitano, Shigehisa
Kagamu, Hiroshi
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Saitama Med Univ, Int Med Ctr, Div Resp Med, 1397-1 Yamane, Hidaka, Saitama 3501298, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
Kagamu, Hiroshi
Yamashita, Makiko
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Canc Inst Hosp JFCR, Ctr Adv Med Dev, Div Canc Immunotherapy Dev, 3-8-31 Ariake, Tokyo 1358550, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
Yamashita, Makiko
Terao, Mayako
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Tokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
Terao, Mayako
Tsuda, Banri
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Tokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
Tsuda, Banri
Okamura, Takuho
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Tokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, JapanTokai Univ, Sch Med, Dept Breast Oncol, 143 Shimokasuya, Isehara, Kanagawa 2591193, Japan
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Univ Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, Brazil
Univ Porto IPATIMUP, Inst Ciencias Biomed Abel Salazar ICBAS, Porto, PortugalUniv Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, Brazil
Lopes-Neto, Belarmino Eugenio
Sousa Nunes-Pinheiro, Diana Celia
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Univ Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, BrazilUniv Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, Brazil
Sousa Nunes-Pinheiro, Diana Celia
Vale Carvalheira, Julio Gil
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Univ Porto IPATIMUP, Inst Ciencias Biomed Abel Salazar ICBAS, Porto, Portugal
Univ Porto IPATIMUP, Ctr Invest Biodiversidade & Recursos Genet CIBIO, Porto, PortugalUniv Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, Brazil
Vale Carvalheira, Julio Gil
Schmitt, Fernando
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Univ Porto IPATIMUP, Fac Med FM, Porto, Portugal
Univ Porto IPATIMUP, Inst Patol & Imunol Mol, Porto, Portugal
Univ Porto UP, Inst Invest & Inovacao Saude I3S, Porto, PortugalUniv Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, Brazil
Schmitt, Fernando
Gartner, Maria de Fatima
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Univ Porto IPATIMUP, Inst Ciencias Biomed Abel Salazar ICBAS, Porto, Portugal
Univ Porto IPATIMUP, Inst Patol & Imunol Mol, Porto, Portugal
Univ Porto UP, Inst Invest & Inovacao Saude I3S, Porto, PortugalUniv Estadual Ceara UECE, Fac Vet FAVET, Programa Posgrad Ciencias Vet PPGCV, Fortaleza, Ceara, Brazil