An intrasplenic injection model of pancreatic cancer metastasis to the liver in mice

被引：10

作者：

O'Brien, Megan ^{[1
,2
]}

Ernst, Matthias ^{[1
,2
]}

Poh, Ashleigh R. ^{[1
,2
]}

机构：

[1] Olivia Newton John Canc Res Inst, Heidelberg, Vic 3084, Australia

[2] La Trobe Univ, Sch Canc Med, Heidelberg, Vic 3084, Australia

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

Cancer; Cell Biology; Cell culture; Immunology; Model Organisms;

D O I：

10.1016/j.xpro.2022.102021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we provide a protocol for an intrasplenic injection model to establish pancreatic tumors in the mouse liver. We describe the steps to inject tumor cells into mouse spleen and to perform a splenectomy, followed by animal recovery and end point analysis of tumors in the liver. This model allows rapid and reproducible tumor growth in a clinically relevant metastatic site, providing a platform to evaluate the efficacy of anti-cancer drugs. This technique can be expanded to other cancer cell lines.For complete details on the use and execution of this protocol, please refer to Poh et al. (2022).1

引用

页数：16

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