Heterologous Expression of the Formicamycin Biosynthetic Gene Cluster Unveils Glycosylated Fasamycin Congeners

被引:5
|
作者
McDonald, Hannah P. [1 ]
Alford, Abigail [1 ]
Devine, Rebecca [1 ]
Hems, Edward S. [1 ]
Nepogodiev, Sergey A. [2 ]
Arnold, Corinne J. [1 ]
Rejzek, Martin [2 ]
Stanley-Smith, Anna [3 ]
Holmes, Neil A. [1 ]
Hutchings, Matthew I. [1 ]
Wilkinson, Barrie [1 ]
机构
[1] John Innes Ctr, Dept Mol Microbiol, Norwich NR4 7UH, England
[2] John Innes Ctr, NMR Platform, Norwich NR4 7UH, England
[3] Isomerase, Cambridge CB10 1XL, England
来源
JOURNAL OF NATURAL PRODUCTS | 2023年 / 86卷 / 07期
基金
英国生物技术与生命科学研究理事会;
关键词
COMPLETE GENOME SEQUENCE; ENGINEERED BIOSYNTHESIS; STREPTOMYCES-COELICOLOR; ESCHERICHIA-COLI; NAPHTHACEMYCINS; ELUCIDATION; POLYKETIDES; RESISTANCE;
D O I
10.1021/acs.jnatprod.3c00052
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Formicamycins and their biosynthetic intermediates thefasamycinsare polyketide antibiotics produced by Streptomyces formicae KY5 from a pathway encoded by the for biosyntheticgene cluster. In this work the ability of Streptomyces coelicolor M1146 and the ability of Saccharopolyspora erythraea & UDelta;ery to heterologously express the for biosynthetic gene cluster were assessed. This led tothe identification of eight new glycosylated fasamycins modified atdifferent phenolic groups with either a monosaccharide (glucose, galactose,or glucuronic acid) or a disaccharide comprised of a proximal hexose(either glucose or galactose), with a terminal pentose (arabinose)moiety. In contrast to the respective aglycones, minimal inhibitoryscreening assays showed these glycosylated congeners lacked antibacterialactivity.
引用
收藏
页码:1677 / 1689
页数:13
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