In Vitro Effect of the Cell-Free Supernatant of the Lactobacillus casei Strain IMAU60214 against the Different Pathogenic of Escherichia coli

被引:6
|
作者
Rocha-Ramirez, Luz Maria [1 ]
Hernandez-Chinas, Ulises [2 ,3 ]
Moreno-Guerrero, Silvia Selene [4 ]
Ramirez-Pacheco, Arturo [4 ]
Eslava, Carlos A. [2 ,3 ]
机构
[1] Hosp Infantil Mexico Dr Federico Gomez, Unidad Invest Enfermedades Infecciosas, Dr Marquez 162, Mexico City 06720, DF, Mexico
[2] Univ Nacl Autonoma Mexico, Unidad Perifer Invest Basica & Clin Enfermedades, Dr Marquez 162, Mexico City 06720, DF, Mexico
[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Salud Publ, Div Invest, Mexico City 04510, DF, Mexico
[4] Hosp Infantil Mexico Dr Federico Gomez, Dept HematoOncol, Dr Marquez 162, Mexico City 06720, DF, Mexico
关键词
Lactobacillus casei IMAU60214; cell-free supernatant; enterohemorrhagic Escherihcia coli enteroaggregative; E; coli; anti-biofilms; coaggregation; anti-cytotoxins; antimicrobial activity; ACIDOPHILUS; PROBIOTICS; VIVO; COAGGREGATION; AGGREGATION; MECHANISMS; SURVIVAL; BIOFILM;
D O I
10.3390/microorganisms11051324
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enteroaggregative Escherichia coli (EAEC) and enterohemorrhagic E. coli (EHEC) are E. coli pathotypes associated with unmanageable diarrhea in children and adults. An alternative to the treatment of infections caused by these microorganisms is the use of the bacteria of the Lactobacillus genus; however, the beneficial effects on the intestinal mucosa are specific to the strain and species. The interest of this study consisted of analyzing the coaggregation properties of Lactobacillus casei IMAU60214, as well as the effect of cell-free supernatant (CSF) on growth and anti-cytotoxic activity in a cell model of the human intestinal epithelium for an agar diffusion assay (HT-29) and the inhibition of biofilm formation on plates of DEC strains of the EAEC and EHEC pathotypes. The results showed that L. casei IMAU60214 exhibits time-dependent coaggregation (35-40%) against EAEC and EHEC that is similar to the control E. coli ATCC 25922. The CSF showed antimicrobial activity (20-80%) against EAEC and EHEC depending on the concentration. In addition, the formation and dispersion of biofilms of the same strains decrease, and the proteolytic pre-treatment with catalase and/or proteinase K (1 mg/mL) of CSF reduces the antimicrobial effect. When evaluating the effect in HT-29 cells pre-treated with CFS on the toxic activity induced by the EAEC and EHEC strains, a reduction of between 30 and 40% was observed. The results show that L. casei IMAU60214 and its CSF have properties that interfere with some properties associated with the virulence of the EAEC and EHEC strains that cause intestinal infection, which supports their use for the control and prevention of infections caused by these bacteria.
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页数:18
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