Quantitative Proteomic Analysis of Cyanide and Mercury Detoxification by Pseudomonas pseudoalcaligenes CECT 5344

被引:2
|
作者
Biello, Karolina A. [1 ]
Olaya-Abril, Alfonso [1 ]
Cabello, Purificacion [2 ]
Rodriguez-Caballero, Gema [1 ]
Saez, Lara P. [1 ]
Moreno-Vivian, Conrado [1 ]
Luque-Almagro, Victor Manuel [1 ]
Roldan, Maria Dolores [1 ]
机构
[1] Univ Cordoba, Dept Bioquim & Biol Mol, Edificio Severo Ochoa,Campus Rabanales, Cordoba, Spain
[2] Univ Cordoba, Dept Bot Ecol & Fisiol Vegetal, Edificio Celestino Mutis,Campus Rabanales, Cordoba, Spain
来源
MICROBIOLOGY SPECTRUM | 2023年 / 11卷 / 04期
关键词
arsenic; biodegradation; cyanide; heavy metals; mercury; Pseudomonas; GENOME SEQUENCE; BACTERIAL; TRANSPORT; MERE;
D O I
10.1128/spectrum.00553-23
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cyanide, mercury, and arsenic are considered very toxic chemicals that are present in nature as cocontaminants in the liquid residues generated by different industrial activities like mining. Considering the huge amounts of toxic cyanide- and mercury-containing wastes generated at a large scale and the high biotechnological potential of P. pseudoalcaligenes CECT 5344 in the detoxification of cyanide present in these industrial wastes, in this work, proteomic, transcriptional, and bioinformatic approaches were used to characterize the molecular response of this bacterium to cyanide and mercury, highlighting the mechanisms involved in the simultaneous detoxification of both compounds. The cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT 5344 uses cyanide and different metal-cyanide complexes as the sole nitrogen source. Under cyanotrophic conditions, this strain was able to grow with up to 100 & mu;M mercury, which was accumulated intracellularly. A quantitative proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been applied to unravel the molecular basis of the detoxification of both cyanide and mercury by the strain CECT 5344, highlighting the relevance of the cyanide-insensitive alternative oxidase CioAB and the nitrilase NitC in the tolerance and assimilation of cyanide, independently of the presence or absence of mercury. Proteins overrepresented in the presence of cyanide and mercury included mercury transporters, mercuric reductase MerA, transcriptional regulator MerD, arsenate reductase and arsenical resistance proteins, thioredoxin reductase, glutathione S-transferase, proteins related to aliphatic sulfonates metabolism and sulfate transport, hemin import transporter, and phosphate starvation induced protein PhoH, among others. A transcriptional study revealed that from the six putative merR genes present in the genome of the strain CECT 5344 that could be involved in the regulation of mercury resistance/detoxification, only the merR2 gene was significantly induced by mercury under cyanotrophic conditions. A bioinformatic analysis allowed the identification of putative MerR2 binding sites in the promoter regions of the regulatory genes merR5, merR6, arsR, and phoR, and also upstream from the structural genes encoding glutathione S-transferase (fosA and yghU), dithiol oxidoreductase (dsbA), metal resistance chaperone (cpxP), and amino acid/peptide extruder involved in quorum sensing (virD), among others.IMPORTANCE Cyanide, mercury, and arsenic are considered very toxic chemicals that are present in nature as cocontaminants in the liquid residues generated by different industrial activities like mining. Considering the huge amounts of toxic cyanide- and mercury-containing wastes generated at a large scale and the high biotechnological potential of P. pseudoalcaligenes CECT 5344 in the detoxification of cyanide present in these industrial wastes, in this work, proteomic, transcriptional, and bioinformatic approaches were used to characterize the molecular response of this bacterium to cyanide and mercury, highlighting the mechanisms involved in the simultaneous detoxification of both compounds. The results generated could be applied for developing bioremediation strategies to detoxify wastes cocontaminated with cyanide, mercury, and arsenic, such as those generated at a large scale in the mining industry.
引用
收藏
页数:18
相关论文
共 43 条
  • [31] Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing
    Wibberg, Daniel
    Bremges, Andreas
    Dammann-Kalinowski, Tanja
    Maus, Irena
    Isabel Igeno, Ma
    Vogelsang, Ralph
    Koenig, Christoph
    Luque-Almagro, Victor M.
    Dolores Roldan, Ma
    Sczyrba, Alexander
    Moreno-Vivian, Conrado
    Blasco, Rafael
    Puehler, Alfred
    Schlueter, Andreas
    JOURNAL OF BIOTECHNOLOGY, 2016, 232 : 61 - 68
  • [32] Quantitative proteomic analysis reveals the mode-of-action for chronic mercury hepatotoxicity to marine medaka (Oryzias melastigma)
    Wang, Minghua
    Wang, Yuyu
    Zhang, Ling
    Wang, Juan
    Hong, Huasheng
    Wang, Dazhi
    AQUATIC TOXICOLOGY, 2013, 130 : 123 - 131
  • [33] Mixotrophic growth of Pseudomonas sp C27 at different C/N ratios: Quantitative proteomic analysis
    Guo, Hongliang
    Chen, Chuan
    Lee, Duu-Jong
    Wang, Aijie
    Ren, Nanqi
    JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS, 2015, 54 : 91 - 95
  • [34] Quantitative proteomic analysis indicates increased synthesis of a quinolone by Pseudomonas aeruginosa isolates from cystic fibrosis airways
    Guina, T
    Purvine, SO
    Yi, EC
    Eng, J
    Goodlett, DR
    Aebersold, R
    Miller, SI
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) : 2771 - 2776
  • [35] Quantitative proteomic analysis reveals proteins involved in the neurotoxicity of marine medaka Oryzias melastigma chronically exposed to inorganic mercury
    Wang, Yuyu
    Wang, Dazhi
    Lin, Lin
    Wang, Minghua
    CHEMOSPHERE, 2015, 119 : 1126 - 1133
  • [36] iTRAQ-based quantitative proteomic analysis of Pseudomonas aeruginosa SJTD-1: A global response to n-octadecane induced stress
    Liu, Huan
    Sun, Wen-Bing
    Liang, Ru-Bing
    Huang, Li
    Hou, Jing-Li
    Liu, Jian-Hua
    JOURNAL OF PROTEOMICS, 2015, 123 : 14 - 28
  • [37] Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes
    Qian, Chen
    Chen, Hongmei
    Johs, Alexander
    Lu, Xia
    An, Jing
    Pierce, Eric M.
    Parks, Jerry M.
    Elias, Dwayne A.
    Hettich, Robert L.
    Gu, Baohua
    PROTEOMICS, 2018, 18 (17)
  • [38] iTRAQ-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain Pseudomonas putida SJTE-1
    Jing Xu
    Lei Zhang
    Jingli Hou
    Xiuli Wang
    Huan Liu
    Daning Zheng
    Rubing Liang
    Scientific Reports, 7
  • [39] iTRAQ-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain Pseudomonas putida SJTE-1
    Xu, Jing
    Zhang, Lei
    Hou, Jingli
    Wang, Xiuli
    Liu, Huan
    Zheng, Daning
    Liang, Rubing
    SCIENTIFIC REPORTS, 2017, 7
  • [40] iTRAQ-based quantitative proteomic analysis reveals potential factors associated with the enhancement of phenazine-1-carboxamide production in Pseudomonas chlororaphis P3
    Xue-Jie Jin
    Hua-Song Peng
    Hong-Bo Hu
    Xian-Qing Huang
    Wei Wang
    Xue-Hong Zhang
    Scientific Reports, 6