Stability of nucleic acid bases in concentrated sulfuric acid: Implications for the habitability of Venus' clouds

What constitutes a habitable planet is a frontier to be explored and requires pushing the boundaries of our terracentric viewpoint for what we deem to be a habitable environment. Despite Venus' 700 K surface temperature being too hot for any plausible solvent and most organic covalent chemistry, Venus' cloud-filled atmosphere layers at 48 to 60 km above the surface hold the main requirements for life: suitable temperatures for covalent bonds; an energy source (sunlight); and a liquid solvent. Yet, the Venus clouds are widely thought to be incapable of supporting life because the droplets are composed of concentrated liquid sulfuric acid-an aggressive solvent that is assumed to rapidly destroy most biochemicals of life on Earth. Recent work, however, demonstrates that a rich organic chemistry can evolve from simple precursor molecules seeded into concentrated sulfuric acid, a result that is corroborated by domain knowledge in industry that such chemistry leads to complex molecules, including aromatics. We aim to expand the set of molecules known to be stable in concentrated sulfuric acid. Here, we show that nucleic acid bases adenine, cytosine, guanine, thymine, and uracil, as well as 2,6-diaminopurine and the "core" nucleic acid bases purine and pyrimidine, are stable in sulfuric acid in the Venus cloud temperature and sulfuric acid concentration range, using UV spectroscopy and combinations of 1D and 2D 1H 13C 15N NMR spectroscopy. The stability of nucleic acid bases in concentrated sulfuric acid advances the idea that chemistry to support life may exist in the Venus cloud particle environment.SignificanceThe search for signs of life beyond Earth is a motivator in modern-day planetary exploration. While our "sister" planet Venus has a surface too hot for life, scientists have speculated that the much cooler atmosphere at 48 to 60 km above the surface might host life in Venus' perpetual cloud cover, as Earth's clouds do. The Venus clouds, however, are composed of concentrated sulfuric acid-an aggressive chemical that destroys most of Earth life's biochemicals and are thought to be sterile to life of any kind. Here, we show that key molecules needed for life (nucleic acid bases) are stable in concentrated sulfuric acid, advancing the notion that the Venus atmosphere environment may be able to support complex chemicals needed for life.