Introduction: Even though HIV testing and care is highly targeted in different strategies in Ethiopia, most sexually active young females do not know their HIV status. Moreover, prior trend analysis has not been conducted among sexually active young females in Ethiopia. Therefore, this study aimed to assess the trend and determinants of HIV testing change among sexually active young females in Material and methods: The current study utilized three consecutive Ethiopia Demographic and Health Survey (EDHS) datasets. A total of 1,201.9, 3,094, and 2,864.7 sexually active female youths were included in 2005, 2011, and 2016 EDHS surveys, respectively. Data were analyzed using Stata version 14.0. Logit-based decomposition analysis was performed to identify contributing factors for HIV testing change, and statistical significance was determined with p-value. Results: The trend of HIV testing increased from 7% in 2005 to 52.5% in 2016 EDHS. The analysis revealed that 19% of the overall change in HIV testing was due to the change in female youths composition. Changes in the composition of young females according to region, age, economic status, age of sexual initiation, and comprehensive HIV/ AIDS knowledge were the major sources of the change. Greater than 80% of the increase in HIV testing was due to differences in coefficient. The change in behaviors of married youths and early starting of sex life were the contributing factors to change of HIV testing over the last decade. Conclusions: Change of HIV testing among sexually active female youths increased in the last 10 years in Ethiopia. However, in order to achieve the HIV-related targets in the country, programmatic interventions targeting uneducated women is needed.