Glutamine Promotes Porcine Intestinal Epithelial Cell Proliferation through the Wnt/β-Catenin Pathway

被引:1
|
作者
Fang, Yong-xia [1 ,2 ]
Lu, En-qing [1 ,2 ]
Cheng, Yu-jie [1 ,2 ]
Xu, E. [1 ,2 ]
Zhu, Min [1 ,2 ]
Chen, Xiang [1 ]
机构
[1] Guizhou Univ, Coll Anim Sci, Lab Anim Genet & Breeding Reprod Plateau Mountaino, Minist Educ, Guiyang 550025, Guizhou, Peoples R China
[2] Guizhou Univ, Inst Anim Nutr & Feed Sci, Guiyang 550025, Peoples R China
基金
中国国家自然科学基金;
关键词
glutamine; porcine intestinal epithelial cells; cell proliferation; Wnt/beta-catenin; TMT proteomics; SIGNALING PATHWAY; BETA-CATENIN; APOPTOSIS; DYSFUNCTION; SURVIVAL; GROWTH; MYC;
D O I
10.1021/acs.jafc.3c08701
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Glutamine (Gln) is a critical nutrient required by neonatal mammals for intestinal growth, especially for newborn piglets. However, the mechanisms underlying the role of Gln in porcine intestinal epithelium development are not fully understood. The objective of the current study was to explore the possible signaling pathway involved in the promotion of porcine intestinal epithelial cell (IPEC-J2) proliferation by Gln. The results showed that 1 mM Gln promoted IPEC-J2 cell proliferation, and tandem mass tag proteomics revealed 973 differentially expressed proteins in Gln-treated IPEC-J2 cells, 824 of which were upregulated and 149 of which were downregulated. Moreover, gene set enrichment analysis indicated that the Wnt signaling pathway is activated by Gln treatment. Western blotting analysis further confirmed that Gln activated the Wnt/beta-catenin signaling pathway. In addition, Gln increased not only cytosolic beta-catenin but also nuclear beta-catenin protein expression. LF3 (a beta-catenin/TCF4 interaction inhibitor) assay and beta-catenin knockdown demonstrated that Gln-mediated promotion of Wnt/beta-catenin signaling and cell proliferation were blocked. Furthermore, the inhibition of TCF4 expression suppressed Gln-induced cell proliferation. These findings further confirmed that Wnt/beta-catenin signaling is involved in the promotion of IPEC-J2 cell proliferation by Gln. Collectively, these findings demonstrated that Gln positively regulated IPEC-J2 cell proliferation through the Wnt/beta-catenin pathway. These data greatly enhance the current understanding of the mechanism by which Gln regulates intestinal development.
引用
收藏
页码:7155 / 7166
页数:12
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