Simple Purification and Antimicrobial Properties of Bacteriocin-like Inhibitory Substance from Bacillus Species for the Biopreservation of Cheese

被引:4
|
作者
Kim, Jong-Hui [1 ]
Lee, Eun-Seon [1 ]
Kim, Bu-Min [1 ]
Ham, Jun-Sang [1 ]
Oh, Mi-Hwa [1 ]
机构
[1] Natl Inst Anim Sci, Anim Prod Utilizat Div, RDA, Wonju 55365, South Korea
关键词
Bacillus; bacteriocin-like inhibitory substance; aqueous two-phase system; AQUEOUS 2-PHASE SYSTEMS; EXTRACTION; GROWTH; NISIN; FOOD;
D O I
10.3390/foods13010010
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Bacteriocins may be used as natural preservatives and antibiotic substitutes in various foods. However, the multistep purification process of bacteriocins results in high production costs, which is an obstacle to their commercial use and consumer accessibility. In this study, a bacteriocin-like inhibitory substance (BLIS) from Bacillus spp. isolated from Korean fermented foods was partially purified using the aqueous two-phase system (ATPS). The maximum activity of the BLIS was achieved for ATPS composed of PEG 1000 (15% [w/w])/ammonium sulfate (20% [w/w])/sodium chloride (2% [w/w]), which caused BLIS activity to increase by 3 times with a 99% recovery rate. In particular, B. amyloliquefaciens Y138-6 BLIS exhibited broad antibacterial activity, high resistance to acid-base stress, and excellent thermal stability. This antibacterial substance inhibited the growth of aerobic bacteria and fungi on the walls of cheese and ripening rooms. These antibacterial properties have been shown to increase food safety and have the potential for use as biopreservatives. Moreover, considering that the execution of the ATPS requires only salts and PEG, it is a simple, environmentally friendly, and cost-effective process and may have industrial applications in the recovery of BLIS from fermentation broth.
引用
收藏
页数:14
相关论文
共 50 条
  • [21] Partial purification and characterization of two bacteriocin-like inhibitory substances produced by bifidobacteria
    Zouhir, Abdelmajid
    Kheadr, Ehab
    Fliss, Ismail
    Ben Hamida, Jeannette
    AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2011, 5 (04): : 411 - 418
  • [22] GLAUCESCIN, A BACTERIOCIN-LIKE SUBSTANCE FROM STREPTOMYCES-GLAUCESCENS
    SCHURTER, W
    KISSLINGABDERHALDEN, M
    LEISINGER, T
    JOURNAL OF GENERAL MICROBIOLOGY, 1979, 113 (AUG): : 243 - 253
  • [23] Bacteriocin-like inhibitory substances from Campylobacter spp.
    Suzanne Nicole Workman
    Floyd E. Been
    Simera R. Crawford
    Marc C. Lavoie
    Antonie van Leeuwenhoek, 2008, 93 : 435 - 436
  • [24] Bacteriocin-like inhibitory substances from Campylobacter spp.
    Workman, Suzanne Nicole
    Been, Floyd E.
    Crawford, Simera R.
    Lavoie, Marc C.
    ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2008, 93 (04): : 435 - 436
  • [25] Purification of a Novel Bacteriocin-Like Inhibitory Substance Produced by Enterococcus faecium ICIS 8 and Characterization of Its Mode of Action
    Vasilchenko, Alexey S.
    Rogozhin, Eugene A.
    Valyshev, Alexander V.
    MICROBIAL DRUG RESISTANCE, 2017, 23 (04) : 447 - 456
  • [26] PRELIMINARY CHARACTERIZATION OF A BACTERIOCIN-LIKE SUBSTANCE PRODUCED BY A BACILLUS SUBTILIS ISOLATED FROM ARGENTINEAN VEGETABLE FOOD
    Fangio, M. F.
    Fritz, R.
    ITALIAN JOURNAL OF FOOD SCIENCE, 2013, 25 (02) : 181 - 188
  • [27] Phylogenetic affiliations of Bacillus amyloliquefaciens isolates produced by a bacteriocin-like substance in goat milk
    Hanafy, Ahmed M.
    Al-Mutairi, Adel A.
    Al-Reedy, Rasha M.
    Al-Garni, Saleh M.
    JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE, 2016, 10 (04): : 631 - 641
  • [28] Isolation and biochemical characterisation of a bacteriocin-like substance produced by Bacillus amyloliquefaciens An6
    Ben Ayed, Hanen
    Maalej, Hana
    Hmidet, Noomen
    Nasri, Moncef
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2015, 3 (04) : 255 - 261
  • [29] INHIBITORY EFFECT OF BACTERIOCIN-LIKE SUBSTANCE FROM ACTINOMYCES-VISCOSUS T14AV
    MACHIGASHIRA, M
    IZUMI, Y
    INOUE, M
    SUEDA, T
    JOURNAL OF DENTAL RESEARCH, 1995, 74 (03) : 978 - 978
  • [30] Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus
    de Souza de Azevedo, Pamela Oliveira
    de Azevedo, Hernando Fernandes
    Figueroa, Elias
    Converti, Attilio
    Manuel Dominguez, Jose
    de Souza Oliveira, Ricardo Pinheiro
    MOLECULAR BIOLOGY REPORTS, 2019, 46 (05) : 4883 - 4891