A novel approach for developing left bundle branch pacing and left bundle branch block in a canine model

Background and ObjectiveLeft bundle branch pacing (LBBP) has shown the benefits in the treatment of dyssynchronous heart failure (HF). The purpose of this study was to develop a novel approach for LBBP and left bundle branch block (LBBB) in a canine model. MethodsA "triangle-center" method by tricuspid valve annulus angiography for LBBP implantation was performed in 6 canines. A catheter was then applied for retrograde His potential recording and left bundle branch (LBB) ablation simultaneously. The conduction system was stained to verify the "triangle-center" method for LBBP and assess the locations of the LBB ablation site in relation to the left septal fascicle (LSF). ResultsThe mean LBB potential to ventricular interval and stimulus-peak left ventricular activation time were 11.8 +/- 1.2 and 35.7 +/- 3.1 ms, respectively. The average intrinsic QRS duration was 44.7 +/- 4.7 ms. LBB ablation significantly prolonged the QRS duration (106.3 +/- 8.3 ms, p < .001) while LBBP significantly shortened the LBBB-QRS duration to 62.5 +/- 5.3 ms (p < .001). After 6 weeks of follow-up, both paced QRS duration (63.0 +/- 5.4 ms; p = .203) and LBBB-QRS duration (107.3 +/- 7.4 ms; p = .144) were unchanged when comparing to the acute phase, respectively. Anatomical analysis of 6 canine hearts showed that the LBBP lead-tip was all placed in LSF area. ConclusionThe new approach for LBBP and LBBB canine model was stable and feasible to simulate the clinical dyssynchrony and resynchronization. It provided a useful tool to investigate the basic mechanisms of underlying physiological pacing benefits.