Low-level outlets are essential flood discharge structures for high-head dams. High-speed free-surface flows releasing into tunnels lead to substantial air discharge, and the air supply is important for gate vibration mitigation, cavitation protection by aeration, and slug flow transition phenomena. An additional challenge is the excessive wind noise due to low air supply efficiency, threatening staff working conditions in flood discharge operations. However, the current understanding of the effects of air vent sizes and locations on the air supply efficiency is still limited. In the present study, based on a series of experimental tests, air vent designs of long flood discharge tunnels, including a single vent and two combined working vents, are analyzed with respect to the air discharge variation. For air vent designs with a small vent size and an installation location downstream, the air discharge into the tunnel is low, and the air supply efficiency is low even for two combined working vents. Equations for the air supply efficiency are proposed, which enables quantifying the ventilation performance, and related vent parameters are recommended for favorable air ventilation designs. The present investigation provides a reference for the flood discharge tunnel design and ventilation operation assessment in hydraulic engineering. Air ventilation design is an important part of flood discharge tunnels and water supply and drainage systems. The single air vent sizes, locations, and combined working vents are considered in the present studies. The single air vent sizes, air vent downstream locations, and two vent distances are effect factors, providing reasonable assessment methods to predict the air supply efficiency for different structural and hydraulic conditions. A reasonable hydraulic design can obtain high air supply efficiency and regulate air discharge distributions in multiple air vents. The guidelines in the present analyses are effective to optimize the multiple air ventilation system and to obtain safety operations and acceptable staff working conditions.